engleski

Genome­wide association study of bitter taste perception of quinine in an isolate population of Croatia

Aim: Taste perception plays an important role in the diet composition, which is the leading lifestyle risk factor for cardiovascular diseases and cancer. The aim of this study was to investigate genetic background of bitter taste perception. Materials and methods: Subjects from the 10, 001 Dalmatians cohort were involved in the bitter taste recognition threshold measurement, based on water solution of quinine, starting from 1E­06 M and increasing by 0.22 log value. A GWAS meta­ analysis was performed using 317.500 SNPs in a sample of 936 individuals from the Island of Korcula and Vis, and the City of Split, Croatia, controlling for age, gender and kindship. Results: Bitter taste threshold was strongly associated with 8 SNPs from PRH1­PRR4, PRH1, TAS2R19 and TAS2R20 gene, with P values between 1.5E­11 to 8.8E­39, belonging to the following SNPs: rs4763602, rs10772420, rs12226919, rs12226920, rs10845279, rs7307031, rs10845289 and rs10492102. Additionally, we detected marginally suggestive SNP at chromosome 6, belonging to ADGRF5 gene (rs1004018 ; P= 4.4E­07). Conclusion: While the finding of the PRH1­PRR4, PRH1, TAS2R19 and TAS2R20 genes confirms the previous studies, the ADGRF5 gene is reported for the first time, suggesting even greater complexity of the genetic basis of bitter taste perception. These results could be used in numerous applications, including personalized dietary planning, fitness and other health­related utilization. Further refinement of these results in the imputed data and increased sample sizes is under way. Funding: Medical Research Council UK, Croatian Science Foundation grant 8875. We gratefully acknowledge contribution from the Institute for Anthropological Research, Croatia.

GWAS, bitter taste, isolate population

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