Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 856200

The transcriptome of herpes simplex virus 1


Zubković, Andreja; Matešić, Marina; Hackenberg, Michael; Kovačević, Lucija; Lisnić, Berislav; Juranić Lisnić, Vanda; Jonjić, Stipan; Vugrek, Oliver; Jurak, Igor
The transcriptome of herpes simplex virus 1 // 6th Croatian Congress of Microbiology with International Participation : book of abstracts / Antolović, Roberto ; Kifer, Domagoj (ur.).
Zagreb: Croatian Microbiological Society, 2016. str. 150-151 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
The transcriptome of herpes simplex virus 1

Autori
Zubković, Andreja ; Matešić, Marina ; Hackenberg, Michael ; Kovačević, Lucija ; Lisnić, Berislav ; Juranić Lisnić, Vanda ; Jonjić, Stipan ; Vugrek, Oliver ; Jurak, Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Croatian Congress of Microbiology with International Participation : book of abstracts / Antolović, Roberto ; Kifer, Domagoj - Zagreb : Croatian Microbiological Society, 2016, 150-151

ISBN
978-953-7778-13-2

Skup
Croatian Congress of Microbiology with International Participatio (6 : 2016)

Mjesto i datum
Sveti Martin na Muri ; Republika Hrvatska, 15.-18.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Herpes simplex virus 1

Sažetak
Herpes simplex virus 1 (HSV-1) is an intensively studied human pathogen for a number of years, however many of its biological properties remain unknown. Although, several HSV-1 strains have been completely sequenced, and partial transcriptomes of the virus have been assigned, the exact transcriptional activity of the virus is yet to be elucidated. Importantly, previous analyses have mainly focused on the transcripts containing open reading frames (ORFs), which might represent a huge underestimation of functional transcripts, namely with regards to recently discovered non-coding RNAs, including regulatory long-non-coding RNAs (lncRNAs) and microRNAs (miRNAs). Several HSV-1 encoded lncRNAs have been detected using traditional techniques, such as Northern blot, and function of most of these have not been elucidated. On the other hand, HSV-1 miRNAs have been discovered very recently and their number is still increasing. For many HSV-1 miRNAs the primary transcripts and the regulation of their promoters are unknown. To generate a comprehensive map of the HSV-1 transcriptome, including the non-coding transcriptome, we analyzed total RNA from cells infected with HSV-1 at different time points using automated NGS library preparation massive parallel sequencing on Illumina platform. We have generated high quality sequence data set (according to FastQC quality control tool) with approx. 25 million reads per sample, and our work in progress shows majority of known virus transcripts. Currently we are trying to map all reads on the virus genome, delineate non-coding transcripts and generate an interactive map of all transcripts. Our specific focus is on the precursors of virus miRNAs, and we investigated thoroughly the potential precursors of miR-H1 and miR-H6 by Northern blot and RACE, which will be now complemented with the transcriptome profile. In addition, we are performing a re-analyses of our miRNA sequencing data sets to evaluate previously and newly discovered miRNA. Of note, many reads obtained by sequencing might falsely satisfy miRNA criteria but represent only the degradation products. We hypothesized that a careful analysis of the transcriptome will also greatly help to define the exact HSV-1 miRNAs.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Ustanove
Medicinski fakultet, Rijeka,
Institut "Ruđer Bošković", Zagreb,
Sveučilište u Rijeci - Odjel za biotehnologiju

Citiraj ovu publikaciju

Zubković, Andreja; Matešić, Marina; Hackenberg, Michael; Kovačević, Lucija; Lisnić, Berislav; Juranić Lisnić, Vanda; Jonjić, Stipan; Vugrek, Oliver; Jurak, Igor
The transcriptome of herpes simplex virus 1 // 6th Croatian Congress of Microbiology with International Participation : book of abstracts / Antolović, Roberto ; Kifer, Domagoj (ur.).
Zagreb: Croatian Microbiological Society, 2016. str. 150-151 (poster, domaća recenzija, sažetak, znanstveni)
Zubković, A., Matešić, M., Hackenberg, M., Kovačević, L., Lisnić, B., Juranić Lisnić, V., Jonjić, S., Vugrek, O. & Jurak, I. (2016) The transcriptome of herpes simplex virus 1. U: Antolović, R. & Kifer, D. (ur.)6th Croatian Congress of Microbiology with International Participation : book of abstracts.
@article{article, year = {2016}, pages = {150-151}, keywords = {Herpes simplex virus 1}, isbn = {978-953-7778-13-2}, title = {The transcriptome of herpes simplex virus 1}, keyword = {Herpes simplex virus 1}, publisher = {Croatian Microbiological Society}, publisherplace = {Sveti Martin na Muri ; Republika Hrvatska} }