Analysis of N-Glycosylation of Total Membrane Proteins (CROSBI ID 57810)
Prilog u knjizi | izvorni znanstveni rad
Podaci o odgovornosti
Pavić, Tamara ; Gornik, Olga
engleski
Analysis of N-Glycosylation of Total Membrane Proteins
Glycosylation of membrane proteins plays a crucial role in various physiological events, including intercellular recognition and intermolecular interactions on the cell surface (Gornik et al., Biochim Biophys Acta 1820:1318– 1326, 2012). To study composition and function of N-glycans on membrane proteins one has to have an efficient and reproducible analytical method, which includes protein extraction and analysis of glycans. In this chapter we provide an analytical approach that includes cloud- point extraction (CPE) of total membrane proteins with the non-ionic detergent Triton X- 114 and subsequent analysis of their N-glycans using hydrophilic interaction liquid chromatography (HILIC)–UPLC/HPLC. The protocol presented here can be used for parallel analysis of both membrane and intracellular proteins.
N-Glycans ; Membrane proteins ; Cloud point extraction ; Triton X-114 ; Protein enrichment ; HILIC-UPLC
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Podaci o prilogu
197-205.
objavljeno
Podaci o knjizi
Lauc, Gordan ; Manfred, Wuhrer
New York (NY): Springer
2017.
978-1-4939-6491-8