Decreasing translation error rate in Escherichia coli increases protein function (CROSBI ID 235219)
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Podaci o odgovornosti
Musa, Marina ; Radman, Miroslav ; Kriško, Anita
engleski
Decreasing translation error rate in Escherichia coli increases protein function
Over-expressed native or recombinant proteins are commonly used for industrial and pharmaceutical purposes, as well as for research. Proteins of interest need to be purified in sufficient quantity, quality and specific activity to justify their commercial price and eventual medical use. Proteome quality was previously positively correlated with ribosomal fidelity, but not on a single protein level. Here, we show that decreasing translational error rate increases the activity of single proteins. In order to decrease the amount of enzyme needed for catalysis, we propose an expression system bearing rpsL141 mutation, which confers high ribosomal fidelity. Using alpha-glucosidase (exo-alpha- 1, 4-glucosidase) and beta-glucanase (beta-D- glucanase) as examples, we show that proteins purified from Escherichia coli bearing rpsL141 mutation have superior activity compared to those purified from wild type E. coli, as well as some commercially available industrial enzymes.
Protein activity ; rpsL141 ; Protein expression systems
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Podaci o izdanju
Povezanost rada
Biotehnologija, Biologija