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Assessment of human telomeric G-quadruplex structures using surface-enhanced Raman spectroscopy


Miljanić, Snežana; Ratkaj, Marina; Matković, Marija; Piantanida, Ivo; Gratteri, Paola; Bazzicalupi, Carla
Assessment of human telomeric G-quadruplex structures using surface-enhanced Raman spectroscopy // Analytical and bioanalytical chemistry, 409 (2017), 9; 2285-2295 doi:10.1007/s00216-016-0172-y (međunarodna recenzija, članak, znanstveni)


Naslov
Assessment of human telomeric G-quadruplex structures using surface-enhanced Raman spectroscopy

Autori
Miljanić, Snežana ; Ratkaj, Marina ; Matković, Marija ; Piantanida, Ivo ; Gratteri, Paola ; Bazzicalupi, Carla

Izvornik
Analytical and bioanalytical chemistry (1618-2642) 409 (2017), 9; 2285-2295

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Surface-enhanced Raman spectroscopy ; G-quadruplex ; human telomere ; silver colloid ; DNA structure

Sažetak
G-quadruplex (G4) structures of a human telomeric 24-mer (5’-TTAGGGTTAGGGTTAGGGTTAGGG-3’) sequence (Tel24) stabilized by sodium and potassium ions have been assessed using surface-enhanced Raman scattering (SERS) spectroscopy. The distinctive SERS spectra of Tel24 in the presence of 100 mM Na+ and 100 mM K+ were obtained and the SERS bands characteristic of the anti-parallel basket-type and the mixed hybrid (3+1) structures, respectively, were identified and assigned. The influence of the SERS active substrate on the scattering enhancement was studied using citrate and chloride covered silver nanoparticles, in the absence and presence of the aggregating agent (0.1 M Na2SO4 and 0.1 M K2SO4). The highly reproducible SERS spectra of Tel24 obtained in various SERS active media indicated the same adsorption mechanism of the cation stabilized G-quadruplexes onto the metal surface, regardless of the silver colloid. The remarkable resemblance between the circular dichroism (CD) spectra of the Tel24 structures with and without the colloid, confirmed that interaction with the enhancing silver surface did not affect stability of the formed G4 structures. The presented study pointed to a great potential of the SERS spectroscopy for the sensitive structural analysis of various G4 topologies.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-1477 - Višenamjensko očitavanje DNA / RNA sekundarne strukture molekularnim kemijskim senzorima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
PLIVA HRVATSKA d.o.o.

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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