engleski

Evaluation of 5-(hydroxymethyl)furan-2- carbaldehyde (HMF) content in honey: comparison of chromatographic and spectrophotometric method

Estimation of honey freshness and storage conditions usually includes determination of 5- (hydroxymethyl)furan-2-carbaldehyde (HMF) content. Maximal HMF content in honey is prescribed by the regulations dealing with honey quality. Therefore, it is important to use proper methods for its determination. The aim of this study was to compare two official methods prescribed by International Honey Commission for determination of HMF content in honey (chromatographic (HPLC) method and spectrophotometric method after White). Method performances were estimated through linearity, precision and accuracy. Later, comparison of methods was performed on 13 honey samples (5 black locust and 8 chestnut honey samples). The results showed that both methods are fit for purpose and comparable within the evaluated range.

HMF content ; honey ; chromatographic method ; spectrophotometric method ; comparison

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