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Pregled bibliografske jedinice broj: 854596

Distributions of Therapeutically Promising Steroids in Cellular Membranes


Riedlova, Kamila; Nekardova, Michaela; Kacer, Petr; Syslova, Kamila; Vazdar, Mario; Jungwirth, Pavel; Kudova, Eva; Cwiklik, Lukasz
Distributions of Therapeutically Promising Steroids in Cellular Membranes // Chemistry and physics of lipids, 203 (2017), 78-86 doi:10.1016/j.chemphyslip.2016.12.004 (međunarodna recenzija, članak, znanstveni)


Naslov
Distributions of Therapeutically Promising Steroids in Cellular Membranes

Autori
Riedlova, Kamila ; Nekardova, Michaela ; Kacer, Petr ; Syslova, Kamila ; Vazdar, Mario ; Jungwirth, Pavel ; Kudova, Eva ; Cwiklik, Lukasz

Izvornik
Chemistry and physics of lipids (0009-3084) 203 (2017); 78-86

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Steroids ; N-Methyl-d-aspartate receptors ; membranes ; molecular dynamics ; chromatography

Sažetak
Interactions of two neurosteroids, inhibiting membrane-bound N-Methyl-d-aspartate receptors, with phospholipid membranes are studied. Namely, endogenous pregnanolone sulfate is compared with pregnanolone glutamate, the latter being a novel synthetic steroidal inhibitor of these receptors with potential pharmaceutical use. Molecular-level details of steroid-phospholipid membranes interactions are scrutinized employing molecular dynamics simulations supported by quantum chemical calculations to assess steroid lipophilicity. Moreover, permeability of both species across membranes is experimentally evaluated by immobilized artificial membrane chromatography. We demonstrate that while there is no significant difference of lipophilicity and membrane permeability between the two sterols, they differ significantly regarding detailed localization in phospholipid membranes. The bulky glutamate moiety of pregnanolone glutamate is flexible and well exposed to the water phase while the sulfate group of pregnanolone sulfate is hidden in the membrane headgroup region. This dissimilarity of behavior in membranes can potentially account for the observed different activities of the two sterols toward membrane-bound N-Methyl-d- aspartate receptors.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Mario Vazdar, (268682)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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