engleski

A dual role of sFRP3 modulating molecule in astrocytic brain tumors

In the present study the expression intensities and localizations of SFRP3 protein across different histopathological grades of astrocytic brain tumors were investigated by immunohistochemistry, digital scanning and image analysis. Our results demonstrated that the differences between expression levels and malignancy grades were statistically significant. Moderate (P=0, 014) and strong (P=0, 028) nuclear expression levels were significantly different with pilocytic and diffuse astrocytomas showed higher expression values. When we divided our sample into two groups, both moderate and high cytoplasmic expression levels were significantly higher in glioblastomas than in the group comprising astrocytomas II and III. We also showed that high grade tumors had lower values of moderate (P=0, 002) and strong nuclear (P=0, 018) expression comparing to low grade tumors. Analysis of cytoplasmic staining showed that strong cytoplasmic expression was significantly higher in the astrocytomas III and glioblastoma group than in astrocytomas I and II group (P=0, 048). Moreover, we found that lower grade astrocytomas had fewer membranous SFRP3 stain than higher grade astrocytomas and that this difference was significant (P=0.036). Our results demonstrated that SFRP3 protein expression levels decreased when located in the nucleus in higher astrocytoma grades, indicating expected behavior as an antagonist of Wnt signaling, whereas when located in the cytoplasm an increase in SFRP3 expression was noted in high grade as compared to low grade astrocytomas. This may suggest that SFRP3 can also act as an agonist of Wnt signaling promoting invasive behavior.

sFRP3 ; astrocytic brain tumors

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