Natural deep eutectic solvents - antimicrobial and cytotoxic (CROSBI ID 643220)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
KRISTINA RADOŠEVIĆ, IVA ČANAK, MARINA CVJETKO BUBALO, KSENIJA MARKOV, JADRANKA FRECE, VIŠNJA GAURINA SRČEK, IVANA RADOJČIĆ REDOVNIKOVIĆ
engleski
Natural deep eutectic solvents - antimicrobial and cytotoxic
Development of green technologies is highly devoted to designing new solvents with good ecotoxicological profle, low cost and desirable technological properties. Natural deep eutectic solvents (NADES) are a new generation of novel alternative solvents. They are mixtures of two or three compounds such as choline chloride, as the cationic salt, and alcohols, acids, amides, amines or sugars as hydrogen-bond donors. NADESs are often presumed to be non-toxic due to natural origin of their constituents, but the evaluation of their toxicity and biodegradability must accompany the research on their synthesis and application. The most of the work reported in the current literature was done on the bacteria and some fungi, while there are only a few scientifc papers publishing results on cytotoxicity of NADESs toward cell lines. Differences in toxicity response between bacteria and cell lines were observed. Most likely, the mechanism of NADES action is associated with its interactions with the cell membranes and therefore could be species- dependent. In the work presented in here we investigated the effect of ten synthesised NADES toward bacteria (Escherichia coli, Proteus mirabilis, Salmonella sp., Pseudomonas aeruginosa, Staphylococcus aureus), yeast Candida albicans and human cell lines (HEK293T, HeLa and MCF-7). Amongst all bacteria evaluated by flter paper diffusion assay, the highest inhibition zone was measured toward Proteus mirabilis, while inhibitory effect was not observed on Candida albicans. Between tested NADESs, high inhibition towards all tested bacteria and yeast was observed with choline chloride:oxalic acid (Ch:OX) and was followed by NADES who also have acidic group as hydrogen-bond donors (malic acid and citric acid). Cytotoxicity toward HEK293T, HeLa and MCF-7 cells was determined by MST assay in the range of concentrations (500 mg ml-1 to 2000 mg ml-1). The highest inhibitory effect on cell growth was observed with Ch:OX which possess moderate cytotoxicity. Other NADES did not caused 50% of growth inhibition in the range of tested concentration so the IC50 value was declared to be > 2000 mg ml-1 i.e. > 5 mM meaning that they possess low cytotoxicity. Overall, our results indicate that is not a good approach to a priori consider NADESs as harmless solvents, simply because most of their forming compounds are benign for humans and the environment. Due to the enormous chemical diversity and huge number of possible combinations, careful and critical assessment of the environmental impact, at least of those NADESs that are likely to have technological application, is needed.
antimicrobial activity; cytotoxicity; natural deep eutectic solvents
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Podaci o prilogu
91-91.
2016.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts of International Conference on Natural Resources, Green Technology & Sustainable Development 2
Ivana Radojčić Redovniković, Kristina Radošević, Tamara Jakovljević, Renata Stojaković, Višnja Gaurina Srček, Dina Erdec Hendrih
Zagreb: Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu
978-953-6893-03-4
Podaci o skupu
International Conference on Natural resources, green technology & sustainable development 2
poster
05.10.2016-07.10.2016
Zagreb, Hrvatska