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Meet the expert 2: Isolation of culture of endothelial, stromal and dendritic cells (CROSBI ID 643067)

Neobjavljeno sudjelovanje sa skupa | neobjavljeni prilog sa skupa

Bulla, Roberta ; Tedesco, Franco ; Juretić, Koraljka ; Laskarin, Gordana Meet the expert 2: Isolation of culture of endothelial, stromal and dendritic cells // The Second EMBIC Summer School- Molecular Mechanisms of Implantation Pečuh, Mađarska, 30.06.2006-04.07.2006

Podaci o odgovornosti

Bulla, Roberta ; Tedesco, Franco ; Juretić, Koraljka ; Laskarin, Gordana

engleski

Meet the expert 2: Isolation of culture of endothelial, stromal and dendritic cells

The suspension of decidual cells was obtained by collagenase digestion and gradient density centrifugation. DEcidual cells were cultured in vitro in the10% RPMI tissue culture medium. Decidual lymphocytes were obtained as non-adherent cell fraction and CD56 molecule was labeled with anti-CD56 monoclonal antibody conjugated with microbeads. Lymphocytes were placed in the magnetic field and allowed to run through the column. The CD56 positive cell fraction was flushed out the column and the purity was assessed (>95%). From CD56 negative fraction CD3 positive lymphocytes were isolated using the same principle. Finaly, from CD3 negative fraction, CD83+ dendritic cells were isolated by the same method.

adherent cells; decidua; lymphocytes; magnetic separation; NK cells; T lymphocytes

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Podaci o prilogu

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Podaci o skupu

The Second EMBIC Summer School- Molecular Mechanisms of Implantation

pozvano predavanje

30.06.2006-04.07.2006

Pečuh, Mađarska

Povezanost rada

Temeljne medicinske znanosti