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Pregled bibliografske jedinice broj: 850032

Manipulation of bioelectrical properties in breast cancer stem cell model


Ester Katja; Marjanović Marko; Guberović Iva; Uzelac Lidija; Mikecin Dražić Ana-Matea; Martin Kleiner Irena; Šumanovac-Ramljak Tatjana; Mlinarić-Majerski Kata; Schreibmayer Wolfgang; Kralj Marijeta
Manipulation of bioelectrical properties in breast cancer stem cell model // Croatian Journal of Oncology Libri Oncologici / Danko Velimir Vrdoljak, Sonja Levanat, Petar Ozretić (ur.).
Zagreb: University Hospital Center Sestre milosrdnice ; University Hospital for Tumors, Zagreb, 2016. str. 31-31 (poster, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 850032 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Manipulation of bioelectrical properties in breast cancer stem cell model

Autori
Ester Katja ; Marjanović Marko ; Guberović Iva ; Uzelac Lidija ; Mikecin Dražić Ana-Matea ; Martin Kleiner Irena ; Šumanovac-Ramljak Tatjana ; Mlinarić-Majerski Kata ; Schreibmayer Wolfgang ; Kralj Marijeta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Croatian Journal of Oncology Libri Oncologici / Danko Velimir Vrdoljak, Sonja Levanat, Petar Ozretić - Zagreb : University Hospital Center Sestre milosrdnice ; University Hospital for Tumors, Zagreb, 2016, 31-31

Skup
HDIR-4: "From bench to Clinic", Fourth Meeting of the Croatian Association for Cancer Research with International Participation

Mjesto i datum
Zagreb, Hrvatska, 3-4.11.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Membrane potential; cancer stem cells; crown ethers

Sažetak
Bioelectricity represents endogenous electrical signaling via ion channels and pumps at the plasma membrane, which shapes unique patterns of voltage potentials across the membranes. Resting membrane potential (Vm) is a functional determinant of cell behaviour. There is a functional relationship between resting membrane potential (Vm) and cell proliferation and differentiation, which can be seen in many cells including normal stem cells and various tumors. Sundelacruz et al have shown that Vm depolarization promotes maintenance of mesenchymal stem cells in an undifferentiated stage. We postulated that depolarized Vm may also help to maintain a population of cancer stem cells (CSCs) in undifferentiated, but highly invasive state. Cancer stem cells (CSCs) represent a subpopulation of cancer cells responsible for tumor formation, relapse and metastasis. The breast CSC model used in this study consists of cells with up-regulated Twist expression (HMLETwist), which are showing markers of CSCs and have functional CSC properties. Therefore, Vm was measured by Nystatin-perforated Patch-Clamp method in a breast CSC model. Also, we measured Vm of SUM159 and MCF7, two breast cancer cell lines with high and low percentage of CSCs among whole population, respectively. Furthermore, we used proprietary potassium ionophores, previously shown to be highly cytotoxic for breast CSC model cells, to modulate membrane potential. Membrane potential after treatment with compounds was estimated using the cationic dye DioC6(3). It was indeed shown that SUM 159 cells are more depolarized than MCF7. Regarding breast CSC model, there was no difference in Vm between HMLEtwist and control HMLE cells, pointing to potential limitations of this model. Two highly cytotoxic proprietary potassium ionophores modulated membrane potential in breast CSC model. Although related by structure, one depolarized, while other hyperpolaryzed cells. Compound 613 causes severe depolarization in HMLEtwist cells. Electrophysiological measurements pointed that compound at first hyperpolarizes cells, which is followed by depolarization. Mode of action of both compounds is under investigation. Measurements of resting potential in CSCs with and without modulators will be further investigated.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-5660 - Mulitidisciplinarni pristup otkriću lijekova s ciljanim djelovanjem na matične stanice tumora – uloga transporta kalija (MultiCaST) (Kralj, Marijeta, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb


Citiraj ovu publikaciju

Ester Katja; Marjanović Marko; Guberović Iva; Uzelac Lidija; Mikecin Dražić Ana-Matea; Martin Kleiner Irena; Šumanovac-Ramljak Tatjana; Mlinarić-Majerski Kata; Schreibmayer Wolfgang; Kralj Marijeta
Manipulation of bioelectrical properties in breast cancer stem cell model // Croatian Journal of Oncology Libri Oncologici / Danko Velimir Vrdoljak, Sonja Levanat, Petar Ozretić (ur.).
Zagreb: University Hospital Center Sestre milosrdnice ; University Hospital for Tumors, Zagreb, 2016. str. 31-31 (poster, domaća recenzija, sažetak, znanstveni)
Ester Katja, Marjanović Marko, Guberović Iva, Uzelac Lidija, Mikecin Dražić Ana-Matea, Martin Kleiner Irena, Šumanovac-Ramljak Tatjana, Mlinarić-Majerski Kata, Schreibmayer Wolfgang & Kralj Marijeta (2016) Manipulation of bioelectrical properties in breast cancer stem cell model. U: Danko Velimir Vrdoljak, Sonja Levanat, Petar Ozretić (ur.)Croatian Journal of Oncology Libri Oncologici.
@article{article, year = {2016}, pages = {31-31}, keywords = {Membrane potential, cancer stem cells, crown ethers}, title = {Manipulation of bioelectrical properties in breast cancer stem cell model}, keyword = {Membrane potential, cancer stem cells, crown ethers}, publisher = {University Hospital Center Sestre milosrdnice ; University Hospital for Tumors, Zagreb}, publisherplace = {Zagreb, Hrvatska} }
@article{article, year = {2016}, pages = {31-31}, keywords = {Membrane potential, cancer stem cells, crown ethers}, title = {Manipulation of bioelectrical properties in breast cancer stem cell model}, keyword = {Membrane potential, cancer stem cells, crown ethers}, publisher = {University Hospital Center Sestre milosrdnice ; University Hospital for Tumors, Zagreb}, publisherplace = {Zagreb, Hrvatska} }




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