engleski

Kinetic Features of Acetylcholinesterase Inhibitor <i>α</i>-terpinolene

Alzheimer's disease (AD) is the most common form of dementia in modern society. It is estimated that there are less than 1% occurrence in population of people between 60 and 64 years old, while there is an exponential increase in older populations. Main symptoms of AD include disruption or loss of higher brain functions (i.e. memory, judgment, thought processes, speech and others). Inhibition of acetylcholinesterase (AchE) enzyme is crucial for ameliorating the symptoms in the first stages of AD and is the only pharmacotherapy to date. The aim of this paper was to test the inhibitory activity of <i>α</i>-terpinolene, naturally occurring monoterpene, on AChE and to determine the type and kinetic parameters of inhibition using various linear plots (i.e. Lineweaver-Burk, Eadie-Hofstee, Hanes- Woolf and Eisenthal – Cornish-Bowden). It was concluded that <i>α</i>-terpinolene exhibits mixed type of inhibition which was shown to be concentration dependent. Also, AChE inhibition kinetic parameters (i.e. K_M, V_max, K_M^app, V_max^app, K_I and K^'_I) were determined.

Alzheimer's disease ; acetylcholinesterase ; <i>α</i>-terpinolene ; kinetics ; inhibition

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