engleski

Comparison of different immunoassays in diagnosis of Epstein-Barr virus infection

Serological testing is the method of choice for the diagnosis of primary Epstein-Barr virus (EBV) infection. Large number of serological tests are in use today. We compared two commercially available enzyme immunoassays for automated EBV serology: Vircell® and Enzygnost®, with final classification of discrepancies by CMIA (chemiluminescent microparticle immunoassay) method (i1000SR Architect Analyzer, Abbott). Collection of 90 sera from clinically suspected infectious mononucleosis cases were analyzed measuring VCA IgM and IgG, and EBNA-1 IgG. The Vircell and Enzygnost approaches gave similar results. Although there were limitations in some individual markers, the assays evaluated are satisfactory for diagnosis of EBV infection.

EBV; serology; immunoassay

supported by "Molecular mechanisms of bacterial pathogenesis and response to stress" (M.A. Grant No. 13.06.1.1.07. from the University of Rijeka, Croatia)