engleski

Horseradish Peroxidase Inhibition by Dipotassium trioxohydroxytetrafluorotriborate, K2[B3O3F4OH] Evaluation of an Electrochemical Method - Chronoamperometry

Most recent in vivo and in vitro studies suggest dipotassium trioxohydroxytetrafluoro-triborate, (K2[B3O3F4OH]), as a promising new therapeutic for cancer diseases. In the present study, this boron heterocyclic compound was investigated as an inhibitor of the horseradish peroxidase (HPR) by electrochemical method. The peroxidase catalyzes the conversion of hydrogen peroxide and the reaction was monitored in the absence and in the presence of K2 [B3O3F4OH]. Electrochemical tests were performed in the classical three- electrode system, using techniques cyclic voltammetry and chronoamperometry. Cyclic voltammetry technique was used to research the influence of different substrate concentration on the activity of the enzyme peroxidase trapped in a layer of Nafion and graphene, immobilized on the GC electrode. For the purpose of these recordings, we have used the following conditions: potential range from -0.7 V to 1.0 V, pH = 7, and a constant potential of 0.9 V was imposed on the working electrode. The kinetics of the reaction was consistent with classical Michaelis-Menten model. By chronoamperometric technique values of Michaelis- Menten constant (Km) were determined and Lineweaver- Burk diagram indicates that this is a non-competitive inhibition.

Boroxine derivative ; peroxidase ; immobilization ; chronoamperometric method

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