engleski

Labelling neural stem cells with different superparamagnetic iron oxide nanoparticles results in change of membrane potential and DNA damage

INTRODUCTION: Biocompatibility, safety and risk assessments of superparamagnetic iron oxide nanoparticles (SPIONs) are of the highest priority in testing their potential applications in biomedicine. Improvement of the biological properties of SPIONs may be achieved by different functionalization and surface modifications. AIMS: This study investigated how different surface functionalizations of SPIONs – uncoated, coated with D-mannose or coated with poly-L-lysine - affected biocompatibility. By using murine neural stem cells (NSCs) as important model system for the regenerative medicine we had a goal to reveal the possible mechanism of toxicity of SPIONs on NSCs, mitochondrial membrane potential, cell membrane potential and DNA damage. RESULTS: We observed hyperpolarization of mitochondrial membrane, dissipated cell membrane potential and increased DNA damages in NSCs, irrespective of the surface coating applied for SPION stabilization. Although surface coating should prevent the toxic effects of SPIONs, our results showed that all of the tested SPION types affected the NSCs similarly indicating that mitochondrial homeostasis is their major cellular target. CONCLUSIONS: Despite the claimed biomedical benefits of SPIONs, the refined determination of their effects on various cellular functions presented in this work highlights the need for further safety evaluations. This investigation helps to fill the knowledge gaps on the criteria that should be considered in evaluating the biocompatibility and safety of novel nanoparticles. ACKNOWLEDGMENTS: This study was supported by EU FP7 grant GlowBrain (REGPOT-2012-CT2012-316120), by EU ESF project MladiMozak (030201-0180) and by the Czech Science Foundation (project no. 16-01128J).

labelling ; neural stem cells ; superparamagnetic iron oxide nanoparticles ; membrane potential ; DNA damage

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