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Pregled bibliografske jedinice broj: 846744

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries


Bulushev, Roman D.; Marion, Sanjin; Petrova, Ekaterina; Davis, Sebastian J.; Maerkl, Sebastian J.; Radenovic, Aleksandra
Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries // Nano letters, 16 (2016), 12; 7882-7890 doi:10.1021/acs.nanolett.6b04165 (međunarodna recenzija, članak, znanstveni)


Naslov
Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

Autori
Bulushev, Roman D. ; Marion, Sanjin ; Petrova, Ekaterina ; Davis, Sebastian J. ; Maerkl, Sebastian J. ; Radenovic, Aleksandra

Izvornik
Nano letters (1530-6984) 16 (2016), 12; 7882-7890

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA−protein complex ; force measurements ; Jarzynski equation ; nanocapillary ; Nanopore ; protein binding site

Sažetak
Through the use of optical tweezers we performed controlled translocations of DNA– protein complexes through nanocapillaries. We used RNA polymerase (RNAP) with two binding sites on a 7.2 kbp DNA fragment and a dCas9 protein tailored to have five binding sites on λ-DNA (48.5 kbp). Measured localization of binding sites showed a shift from the expected positions on the DNA that we explained using both analytical fitting and a stochastic model. From the measured force versus stage curves we extracted the nonequilibrium work done during the translocation of a DNA–protein complex and used it to obtain an estimate of the effective charge of the complex. In combination with conductivity measurements, we provided a proof of concept for discrimination between different DNA–protein complexes simultaneous to the localization of their binding sites.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA


Ustanove
Institut za fiziku, Zagreb

Autor s matičnim brojem:
Sanjin Marion, (330800)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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