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Pregled bibliografske jedinice broj: 843187

Effects of Diclofenac, L-NAME, L-arginine, and Pentadecapeptide BPC 157 on Gastrointestinal, Liver, and Brain Lesions, Failed Anastomosis, and Intestinal Adaptation Deterioration in 24 hour-short-bowel rats


Lojo, Nermin; Rašić, Žarko; Zenko Sever, Anita; Kolenc, Danijela; Vukušić, Darko; Drmić, Domagoj; Zoričić, Ivan; Sever, Marko; Seiwerth, Sven; Sikiric, Predrag
Effects of Diclofenac, L-NAME, L-arginine, and Pentadecapeptide BPC 157 on Gastrointestinal, Liver, and Brain Lesions, Failed Anastomosis, and Intestinal Adaptation Deterioration in 24 hour-short-bowel rats // PLoS One, 11(9) (2016), e0162590. doi:10.1371/journal.pone.0162590 (međunarodna recenzija, članak, znanstveni)


Naslov
Effects of Diclofenac, L-NAME, L-arginine, and Pentadecapeptide BPC 157 on Gastrointestinal, Liver, and Brain Lesions, Failed Anastomosis, and Intestinal Adaptation Deterioration in 24 hour-short-bowel rats

Autori
Lojo, Nermin ; Rašić, Žarko ; Zenko Sever, Anita ; Kolenc, Danijela ; Vukušić, Darko ; Drmić, Domagoj ; Zoričić, Ivan ; Sever, Marko ; Seiwerth, Sven ; Sikiric, Predrag

Izvornik
PLoS One (1932-6203) 11(9) (2016), E0162590;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
BPC 157; diclofenac; small intestine resection; NO system; rats

Sažetak
Stable gastric pentadecapeptide BPC 157 was previously used to ameliorate wound healing following major surgery and counteract diclofenac toxicity. To resolve the increasing early risks following major massive small bowel resectioning surgery, diclofenac combined with nitric oxide (NO) system blockade was used, suggesting therapy with BPC 157 and the nitric oxide synthase (NOS substrate) L-arginine, is efficacious. Immediately after anastomosis creation, short-bowel rats were untreated or administered intraperitoneal diclofenac (12 mg/kg), BPC 157 (10 µg/kg or 10 ng/kg), L-NG-nitroarginine methyl ester (L-NAME, 5 mg/kg), L-arginine (100 mg/kg) alone or combined, and assessed 24 h later. Short-bowel rats exhibited poor anastomosis healing, failed intestine adaptation, and gastrointestinal, liver, and brain lesions, which worsened with diclofenac. This was gradually ameliorated by immediate therapy with BPC 157 and L-arginine. Contrastingly, NOS-blocker L-NAME induced further aggravation and lesions gradually worsened. Specifically, rats with surgery alone exhibited mild stomach/duodenum lesions, considerable liver lesions, and severe cerebral/hippocampal lesions while those also administered diclofenac showed widespread severe lesions in the gastrointestinal tract, liver, cerebellar nuclear/Purkinje cells, and cerebrum/hippocampus. Rats subjected to surgery, diclofenac, and L-NAME exhibited the mentioned lesions, worsening anastomosis, and macro/microscopical necrosis. Thus, rats subjected to surgery alone showed evidence of deterioration. Furtheremore, rats subjected to surgery and administered diclofenac showed worse symptoms, than the rats subjected to surgery alone did. Rats subjected to surgery combined with diclofenac and L-NAME showed the worst deterioration. Rats subjected to surgery exhibited habitual adaptation of the remaining small intestine, which was markedly reversed in rats subjected to surgery and diclofenac, and those with surgery, diclofenac, and L-NAME. BPC 157 completely ameliorated symptoms in massive intestinal resection-, massive intestinal resection plus diclofenac-, and massive intestinal resection plus diclofenac plus L-NAME-treated short bowel rats that presented with cyclooxygenase (COX)-NO-system inhibition. L-arginine ameliorated only L-NAME-induced aggravation of symptoms in rats subjected to massive intestinal resection and administered diclofenac plus L-NAME.

Izvorni jezik
Engleski

Napomena
Projekt MZOS 108-1083570-3636 greškom nije naveden u radu. Voditelj projekta je profesor Žarko Rašić



POVEZANOST RADA


Projekt / tema
108-1080321-0389 - Utjecaj pentadekapeptida BPC 157 na ženski urogenitalni sustav (Hrvoje Vrčić, )
108-1083570-3635 - Pentadekapeptid BPC 157 - daljnja istraživanja (Predrag Sikirić, )
108-1083570-3636 - Učinak BPC 157 na induciranu bilijarnu opstrukciju (Tomislav Anić, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Zoological Record


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