Genotoxic effects of glyphosate in human derived hepatoma (HepG2) cells assessed by cytome assay (CROSBI ID 640868)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Kašuba, Vilena ; Milić, Mirta ; Kopjar, Nevenka ; Mladinić, Marin ; Želježić, Davor
engleski
Genotoxic effects of glyphosate in human derived hepatoma (HepG2) cells assessed by cytome assay
Glyphosate is the most widely used nonselective herbicide. Until recently, it was considered environmentally safe and minimally toxic to humans. Recent studies suggest that glyphosate and its metabolites could affect normal human cell development. In this study, glyphosate was tested at concentrations of 0.5, 2.91, and 3.5 μg mL-1, which corresponded to the values of acceptable daily intake (ADI), residential exposure level (REL), and occupational exposure level (OEL). Analyses of micronuclei, nuclear buds, nucleoplasmic bridges, apoptosis/necrosis, and nuclear division index in HepG2 cells were performed following 4 and 24 hours of in vitro treatment. The results demonstrated that 4-hour exposure to glyphosate slightly increased cytogenetic damage in terms of micronuclei, statistically significant only at OEL. Despite a nonsignificant increase in micronuclei frequencies at ADI and REL, a significant increase in nuclear bud frequency was found. Since nuclear budding represents a micronuclei formation mechanism, our results indicate that even a low dose such as ADI can influence the DNA/cytogenetic damage level. After 24h of exposure, a lower number of binucleated cells was found at all of the three tested concentrations compared to control. Nuclear bud frequency changed only in the ADI sample, while in the OEL- and REL-treated samples, it was significantly lower. The control and treated cells did not significantly differ in the number of nucleoplasmic bridges. It is possible that the two exposure times selected in this study were too short for a reliable estimation of glyphosate genotoxicity, which has to be further clarified through other in vitro/ in vivo models. This work was financially supported by Project No.8366 Organic Pollutants in Environment – Markers and Biomarkers of Toxicity (OPENTOX), funded by the Croatian Science Foundation.
cytome assay ; genotoxic effect ; glyphosate ; human derived hepatoma cells
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Podaci o prilogu
44-44.
2016.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Arhiv za higijenu rada i toksikologiju
Durgo, Ksenija
Zagreb: Institut za medicinska istraživanja i medicinu rada
0004-1254
1848-6312
Podaci o skupu
5th Croatian Congress of Toxicology with International Participation
poster
09.10.2016-12.10.2016
Poreč, Hrvatska
Povezanost rada
Javno zdravstvo i zdravstvena zaštita, Kliničke medicinske znanosti