Naslov
Parameters of oxidative stress in the organs of adult sham-operated and gonadectomised male and female rats

Autori
Rašić, Dubravka ; Micek, Vedran ; Breljak, Davorka ; Vrhovac Madunić, Ivana ; Karaica, Dean ; Novak Jovanović, Ivana ; Gerić, Marko ; Gajski, Goran ; Jurasović, Jasna ; Ljubojević, Marija ; Nanić, Lucia ; Orct, Tatjana ; Rubelj, Ivica ; Sabolić, Ivan ; Peraica, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Ahiv za higijenu rada i toksikologiju Supplement / Durgo, Ksenija - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2016, 36-36

Skup
5th Croatian Congress of Toxicology with International Participation

Mjesto i datum
Poreč, Hrvatska, 9.-12.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
aging; glutathione; malondialdehyde; sex hormones

Sažetak
Aging is a physiological process characterized by a reduced ability to respond to environmental stressors. Despite many aging theories, this process is still poorly understood. As evidenced in recent studies, aging may be associated with increased oxidative stress (OS), which may affect an organ’s functional and morphological characteristics. Reactive oxygen species can change the structure and function of membrane lipids, inhibit enzyme activities and oxidative phosphorylation, and oxidize nucleic acids and proteins. This study used gonadectomised rats (N=5) as an experimental model of aging and sham-operated rats as the controls to investigate if sex hormones affect OS parameters in the major internal organs. Experiments were performed on three-month old Wistar rats of both sexes six weeks after the surgical procedures. Glutathione (GSH) was measured in the kidneys, liver, brain and plasma. Significant difference in GSH concentration was observed only in plasma of sham-operated females (3.62±0.34 nmol g-1 tissue) compared to sham-operated males (4.93±0.68 nmol g-1 tissue, mean±SD ; P<0.05). Ovariectomy in females and castration in males had no effect on GSH concentration in the liver or kidneys. Malondialdehyde (MDA) concentration was measured in the kidneys, liver, brain, plasma and urine. Significant changes (P<0.05) were noted only in sham-operated rats related to sex with the levels (mean±SD) in the liver higher in females than in males (44.6±9.02 vs. 30.7±3.92 nmol g-1 tissue) ; and in urine higher in males than in females (6.67±1.58 vs. 4.98±1.46 µmol L-1). These results indicate that sex hormones have a limited influence on measured OS parameters in the rat internal organs.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA