Pregled bibliografske jedinice broj: 841113
Parameters of oxidative stress in the organs of adult sham-operated and gonadectomised male and female rats
Parameters of oxidative stress in the organs of adult sham-operated and gonadectomised male and female rats // Ahiv za higijenu rada i toksikologiju Supplement / Durgo, Ksenija (ur.).
Zagreb: Institut za medicinska istraživanja i medicinu rada, 2016. str. 36-36 (poster, domaća recenzija, sažetak, znanstveni)
Naslov
Parameters of oxidative stress in the organs of adult sham-operated and gonadectomised male and female rats
Autori
Rašić, Dubravka ; Micek, Vedran ; Breljak, Davorka ; Vrhovac Madunić, Ivana ; Karaica, Dean ; Novak Jovanović, Ivana ; Gerić, Marko ; Gajski, Goran ; Jurasović, Jasna ; Ljubojević, Marija ; Nanić, Lucia ; Orct, Tatjana ; Rubelj, Ivica ; Sabolić, Ivan ; Peraica, Maja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Ahiv za higijenu rada i toksikologiju Supplement
/ Durgo, Ksenija - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2016, 36-36
Skup
5th Croatian Congress of Toxicology with International Participation
Mjesto i datum
Poreč, Hrvatska, 9.-12.10.2016
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Aging; glutathione; malondialdehyde; sex hormones
Sažetak
Aging is a physiological process characterized by a reduced ability to respond to environmental stressors. Despite many aging theories, this process is still poorly understood. As evidenced in recent studies, aging may be associated with increased oxidative stress (OS), which may affect an organ’s functional and morphological characteristics. Reactive oxygen species can change the structure and function of membrane lipids, inhibit enzyme activities and oxidative phosphorylation, and oxidize nucleic acids and proteins. This study used gonadectomised rats (N=5) as an experimental model of aging and sham-operated rats as the controls to investigate if sex hormones affect OS parameters in the major internal organs. Experiments were performed on three-month old Wistar rats of both sexes six weeks after the surgical procedures. Glutathione (GSH) was measured in the kidneys, liver, brain and plasma. Significant difference in GSH concentration was observed only in plasma of sham-operated females (3.62±0.34 nmol g-1 tissue) compared to sham-operated males (4.93±0.68 nmol g-1 tissue, mean±SD ; P<0.05). Ovariectomy in females and castration in males had no effect on GSH concentration in the liver or kidneys. Malondialdehyde (MDA) concentration was measured in the kidneys, liver, brain, plasma and urine. Significant changes (P<0.05) were noted only in sham-operated rats related to sex with the levels (mean±SD) in the liver higher in females than in males (44.6±9.02 vs. 30.7±3.92 nmol g-1 tissue) ; and in urine higher in males than in females (6.67±1.58 vs. 4.98±1.46 µmol L-1). These results indicate that sex hormones have a limited influence on measured OS parameters in the rat internal organs.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekt / tema
HRZZ-IP-2013-11-1481 - Starosno-ovisna ekspresija membranskih prijenosnika u štakora (Ivan Sabolić, )
Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb
Autor s matičnim brojem:
Dubravka Rašić, (296751)
Davorka Breljak, (218831)
Ivana Vrhovac Madunić, (327435)
Dean Karaica, (333825)
Ivana Novak Jovanović, (268215)
Marko Gerić, (327402)
Goran Gajski, (296740)
Jasna Jurasović, (160496)
Marija Ljubojević, (230483)
Tatjana Orct, (335980)
Ivica Rubelj, (108443)
Ivan Sabolić, (64826)
Maja Peraica, (113106)
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- Scopus
- MEDLINE