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Pregled bibliografske jedinice broj: 84111

Shortening adenovirus type 5 fiber shaft decreases the efficiency of post binding steps in CAR-expressing and non-expressing cells


Ambriović-Ristov, Andreja; Mercier, Stephanie; Eloit, Marc
Shortening adenovirus type 5 fiber shaft decreases the efficiency of post binding steps in CAR-expressing and non-expressing cells // Virology, 312 (2003), 425-433 (međunarodna recenzija, članak, znanstveni)


Naslov
Shortening adenovirus type 5 fiber shaft decreases the efficiency of post binding steps in CAR-expressing and non-expressing cells

Autori
Ambriović-Ristov, Andreja ; Mercier, Stephanie ; Eloit, Marc

Izvornik
Virology (0042-6822) 312 (2003); 425-433

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Adenovirus type 5; fiber; shaft length; attachment; internalization

Sažetak
The coxsackie B virus and adenovirus receptor (CAR) functions as an attachment receptor for multiple adenovirus serotypes. It has been shown that apart from virus-cellular receptor interactions, the fiber shaft length also influences viral tropism. We therefore generated Ad5Fb 639 virus with 8 repeats in the shaft, instead of the 22 repeats present in the wild type. Here, we show that the extent of attachment of the virus with shortened fiber to CAR-expressing cells was 3 to 5 fold lower than that of the wild type. Transduction studies, however, clearly showed that infection of CAR-expressing cells with Ad5Fb 639 was strongly impaired by comparison with the wild type virus. Since this impairment was not linked to a proportional reduction in binding to cells, it appeared to be linked to subsequent/later events in infection. A similar decrease in efficacy of post binding steps was also evidenced in cells that did not express CAR.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
0098076

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Andreja Ambriović Ristov, (173311)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE