engleski

Shortening adenovirus type 5 fiber shaft decreases the efficiency of post binding steps in CAR-expressing and non-expressing cells

The coxsackie B virus and adenovirus receptor (CAR) functions as an attachment receptor for multiple adenovirus serotypes. It has been shown that apart from virus-cellular receptor interactions, the fiber shaft length also influences viral tropism. We therefore generated Ad5Fb 639 virus with 8 repeats in the shaft, instead of the 22 repeats present in the wild type. Here, we show that the extent of attachment of the virus with shortened fiber to CAR-expressing cells was 3 to 5 fold lower than that of the wild type. Transduction studies, however, clearly showed that infection of CAR-expressing cells with Ad5Fb 639 was strongly impaired by comparison with the wild type virus. Since this impairment was not linked to a proportional reduction in binding to cells, it appeared to be linked to subsequent/later events in infection. A similar decrease in efficacy of post binding steps was also evidenced in cells that did not express CAR.

adenovirus type 5; fiber; shaft length; attachment; internalization

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