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Pregled bibliografske jedinice broj: 840160

Effect of magnesium ions on the structure of DNA thin films: an infrared spectroscopy study


Serec, Kristina; Dolanski Babić, Sanja; Podgornik, Rudolf; Tomić, Silvia
Effect of magnesium ions on the structure of DNA thin films: an infrared spectroscopy study // Nucleic acids research, 44 (2016), 17; 8456-8464 doi:10.1093/nar/gkw696 (međunarodna recenzija, članak, znanstveni)


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Naslov
Effect of magnesium ions on the structure of DNA thin films: an infrared spectroscopy study

Autori
Serec, Kristina ; Dolanski Babić, Sanja ; Podgornik, Rudolf ; Tomić, Silvia

Izvornik
Nucleic acids research (0305-1048) 44 (2016), 17; 8456-8464

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA thin films ; vibrational and structural features ; FTIR ; electrostatic interactions ; phase transition to compact form

Sažetak
Utilizing Fourier transform infrared spectroscopy we have investigated the vibrational spectrum of thin dsDNA films in order to track the structural changes upon addition of magnesium ions. In the range of low magnesium concentration ([magnesium]/[phosphate] = [Mg]/[P] < 0.5), both the red shift and the intensity of asymmetric PO2 stretching band decrease, indicating an increase of magnesium-phosphate binding in the backbone region. Vibration characteristics of the A conformation of the dsDNA vanish, whereas those characterizing the B conformation become fully stabilized. In the crossover range with comparable Mg and intrinsic Na DNA ions ([Mg]/[P] ≈ 1) B conformation remains stable ; vibrational spectra show moderate intensity changes and a prominent blue shift, indicating a reinforcement of the bonds and binding in both the phosphate and the base regions. The obtained results reflect the modified screening and local charge neutralization of the ds-DNA backbone charge, thus consistently demonstrating that the added Mg ions interact with DNA via long-range electrostatic forces. At high Mg contents ([Mg]/[P] > 10), the vibrational spectra broaden and show a striking intensity rise, while the base stacking remains unaffected. We argue that at these extreme conditions, where a charge compensation by vicinal counterions reaches 92–94%, DNA may undergo a structural transition into a more compact form.

Izvorni jezik
Engleski

Znanstvena područja
Fizika



POVEZANOST RADA


Ustanove:
Institut za fiziku, Zagreb,
Medicinski fakultet, Zagreb

Profili:

Avatar Url Silvia Tomić (autor)

Avatar Url Kristina Serec (autor)

Avatar Url Sanja Dolanski-Babić (autor)

Citiraj ovu publikaciju

Serec, Kristina; Dolanski Babić, Sanja; Podgornik, Rudolf; Tomić, Silvia
Effect of magnesium ions on the structure of DNA thin films: an infrared spectroscopy study // Nucleic acids research, 44 (2016), 17; 8456-8464 doi:10.1093/nar/gkw696 (međunarodna recenzija, članak, znanstveni)
Serec, K., Dolanski Babić, S., Podgornik, R. & Tomić, S. (2016) Effect of magnesium ions on the structure of DNA thin films: an infrared spectroscopy study. Nucleic acids research, 44 (17), 8456-8464 doi:10.1093/nar/gkw696.
@article{article, year = {2016}, pages = {8456-8464}, DOI = {10.1093/nar/gkw696}, keywords = {DNA thin films, vibrational and structural features, FTIR, electrostatic interactions, phase transition to compact form}, journal = {Nucleic acids research}, doi = {10.1093/nar/gkw696}, volume = {44}, number = {17}, issn = {0305-1048}, title = {Effect of magnesium ions on the structure of DNA thin films: an infrared spectroscopy study}, keyword = {DNA thin films, vibrational and structural features, FTIR, electrostatic interactions, phase transition to compact form} }
@article{article, year = {2016}, pages = {8456-8464}, DOI = {10.1093/nar/gkw696}, keywords = {DNA thin films, vibrational and structural features, FTIR, electrostatic interactions, phase transition to compact form}, journal = {Nucleic acids research}, doi = {10.1093/nar/gkw696}, volume = {44}, number = {17}, issn = {0305-1048}, title = {Effect of magnesium ions on the structure of DNA thin films: an infrared spectroscopy study}, keyword = {DNA thin films, vibrational and structural features, FTIR, electrostatic interactions, phase transition to compact form} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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