engleski

Proteomic responses of Nicotiana tabacum seedlings exposed to differently coated silver nanoparticles

Interest in small sized (1-100 nm) nanoparticles (NPs) is growing rapidly. However, the same characteristics making them so attractive for exploitation in new products have led to concerns that NPs may pose a risk to the environment and human health. Among different available NPs, silver nanoparticles (AgNPs) are of particular interest because of their well-known antibacterial and antifungal properties and can be found in a variety of consumer products. Since plants play a significant role in accumulation and biodistribution of many environmentally released substances, they could be influenced by AgNPs, serving as a potential pathway for AgNP-transport and bioaccumulation into food chains. Current proteomics-based techniques have been scarcely used on plants to analyse the effects of AgNPs. Our research goal was to determine the influence of differently coated AgNPs on tobacco seedlings proteome. Seedlings were grown from seeds in a liquid ½ MS medium supplemented with 100 μM citrate-, PEG- and bPEI-AgNP. After 10 days proteins were extracted from whole seedlings and analysed with MALDI-TOF/TOF. MS spectra were identified with MASCOT and analysed with R statistical and proteomics packages. Here we present changes in proteomes of tobacco seedlings upon AgNP treatments and discuss differences in proteome response dependent on different AgNP-coatings used.

silver nanoparticles; proteomics; tobacco seedlings

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