engleski

Determination of (2E)-10-hydroxydec-2-enoic acid content in royal jelly

Health-promoting properties of royal jelly are known for many years and the scientific evidences are increasing every day. Many of them are attributed to the presence of (2E)-10-hydroxydec-2-enoic acid (10-HDA) in royal jelly. The content of this fatty acid, present only in royal jelly is considered as royal jelly freshness and adulteration indicator. A few years ago, guidelines for royal jelly quality specifications are proposed but till today, there is neither international standard for royal jelly quality nor harmonised method for 10-HDA content determination. Chromatographic (HPLC) method with photo-diode array (PDA) detection was validated and used for determination of 10-HDA content in royal jelly samples. 10-HDA identification was performed based on the retention time and comparison of 10-HDA absorbance spectrum of royal jelly samples with spectrum of standard, while internal standard method was used for quantification. Method validation was assessed through linearity, precision and accuracy and found to be fit for purpose. 10-HDA content determined in five fresh royal jelly samples ranged from 1.56 % to 3.78 %. The guidelines for royal jelly quality specifications proposed a lower limit of 10-HDA to 1.0% and based on the obtained results, the samples used in this study can be considered fresh and genuine.

royal jelly ; 10-HDA content ; HPLC method

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