engleski

Assessment of the influence of listerine cool mint mouthwash on buccal cells measured by buccal micronucleus cytome assay

Listerine is worldwide the most used brand of mouthwash for oral hygiene maintenance. Listerine Cool Mint mouthwash is made from four herbal extracts: thymol, menthol, eucalyptol, and methyl salicylate in a solution of 21.6 % of ethanol. All these individual ingredients can cause cell damage. Buccal epithelial cells are in direct contact with the mouthwash and their analysis using the buccal micronucleus cytome assay (BMCyt) can show the influence of mouthwash on the differentiation (frequency of basal, differentiated, binuclear cells, and apoptosis phases: condensed chromatin, karriorhectic, pyknotic, and karyolitic cells) or genomic stability of buccal cells (frequency of micronucleus (MN), nuclear buds or structures called broken eggs). Ten healthy individuals used mouthwash twice per day during a two-week treatment, with sample collection before and after the treatment. The treatment did not significantly influence cell differentiation or genomic instability, although MN frequency was higher after the treatment (1 vs. 1.5). Individual differences were found and consumers of strong alcoholic drinks had a higher MN frequency when compared to other individuals. Following experiments should be performed on a greater number of individuals and specific groups, such as individuals who regularly consume alcohol, to analyse whether a synergistic influence of mouthwash with an additional source of ethanol exists and to discover if this population runs a higher risk of genomic instability. BMCyt has demonstrated its sensitivity in evaluating changes before and after the treatment, and it has been used for the first time in Croatia as well as on Listerine Cool Mint mouthwash

buccal cells ; buccal micronucleus cytome assay ; genome damage ; Listerine Cool Mint mouthwash

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