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Pregled bibliografske jedinice broj: 838055

Bilateral antinociceptive effect of botulinum toxin type A in a rat model of „mirror“ pain involves spinal GABA


Drinovac, Višnja; Bach-Rojecky, Lidija; Lacković, Zdravko
Bilateral antinociceptive effect of botulinum toxin type A in a rat model of „mirror“ pain involves spinal GABA // 9th Congress of the European Pain Federation EFIC
Beč, Austrija, 2015. (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
Bilateral antinociceptive effect of botulinum toxin type A in a rat model of „mirror“ pain involves spinal GABA

Autori
Drinovac, Višnja ; Bach-Rojecky, Lidija ; Lacković, Zdravko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
9th Congress of the European Pain Federation EFIC

Mjesto i datum
Beč, Austrija, 02-05.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Botulinum toxin A; Mirror pain; GABA-A receptor; bicuculline

Sažetak
Background and aims: According to recent observations botulinum toxin type A (BTX-A) alleviates different types of pain by acting within central nervous system, but the mechanism of its central action is poorly understood. Previously we reported the bilateral antinociceptive effect of BTX-A in carrageenan induced bilateral muscle pain. Here we investigated the effects of selective GABA antagonist bicuculline on BTX-A’s bilateral antinociceptive effect. Methods: Male Wistar rats were injected into right gastrocnemius muscle with 100 μL of 3% carrageenan or saline. Animals which developed bilateral secondary mechanical allodynia (tested with von Frey filaments) were divided into groups (6 animals per group): saline or BTX-A (5 U/kg) (intraplantar, into the right hind paw) ; saline or BTX-A + bicuculline (1 µg/ 10 µL, intrathecal) ; saline or BTX-A + bicuculline (1 µg/ 10 µL, into cisterna magna). BTX-A was injected 5 days and bicuculline 30 min before nociceptive testing. Results: Unilaterally injected BTX-A decreased mechanical hypersensitivity not only on ipsilateral (p<0.001), but on the contralateral side as well (p<0.001). Intrathecal, low dose of bicuculline abolished the bilateral antinociceptive effect in BTX-A treated animals (p<0.001), while the same dose applied into cisterna magna had no effect on BTX-A’s antinociceptive action. Bicuculline alone had no effect on carrageenan induced bilateral allodynia. Conclusions: Unilaterally injected BTX-A has bilateral antinociceptive effect in carrageenan induced bilateral pain that can be prevented with GABA antagonist bicuculline. This indicates the involvement of central GABA-ergic system in bilateral antinociceptive effect of BTX-A, most probably at spinal level.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Zagreb


Citiraj ovu publikaciju

Drinovac, Višnja; Bach-Rojecky, Lidija; Lacković, Zdravko
Bilateral antinociceptive effect of botulinum toxin type A in a rat model of „mirror“ pain involves spinal GABA // 9th Congress of the European Pain Federation EFIC
Beč, Austrija, 2015. (poster, međunarodna recenzija, sažetak, znanstveni)
Drinovac, V., Bach-Rojecky, L. & Lacković, Z. (2015) Bilateral antinociceptive effect of botulinum toxin type A in a rat model of „mirror“ pain involves spinal GABA. U: 9th Congress of the European Pain Federation EFIC.
@article{article, year = {2015}, keywords = {Botulinum toxin A, Mirror pain, GABA-A receptor, bicuculline}, title = {Bilateral antinociceptive effect of botulinum toxin type A in a rat model of „mirror“ pain involves spinal GABA}, keyword = {Botulinum toxin A, Mirror pain, GABA-A receptor, bicuculline}, publisherplace = {Be\v{c}, Austrija} }
@article{article, year = {2015}, keywords = {Botulinum toxin A, Mirror pain, GABA-A receptor, bicuculline}, title = {Bilateral antinociceptive effect of botulinum toxin type A in a rat model of „mirror“ pain involves spinal GABA}, keyword = {Botulinum toxin A, Mirror pain, GABA-A receptor, bicuculline}, publisherplace = {Be\v{c}, Austrija} }




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