engleski

In vitro generation of potentially source of insulin-secreting structures

Stem cells are self-renewing elements that can generate the many cell types in the body. These cells are derived from an early stage of the embryo and are named embryonic stem (ES) ce11s. ES ce11s isolated from 129/sv mice can be differentiate into insulin-producing ce11s (Lumelsky et al, 2001). Because of numerous patients with Diabetes mellitus , transplantation of in vitro produced Langerhans islets causes successful protection from development of diabetic late complication. When injected into diabetic mice, these ce11s undergo rapid vascularization and maintain a clustered, islet-like organization. There is no data about such work on NOD (non-obesity) mice, which spontaneously developed Diabetes melltus .The aim of our work was generated structure like this from blastocysts of NOD mice. The procedure involved some steps and until today we successful generated embryoid bodies, which presents the base levels for expansion of insulin-producing cells. Blastocysts are isolated from uterus of 3.5 days pregnant NOD mice. After 48 hours of culturing the zona pellucida was dissolved in acid media. Inner ce11 mass of blastocysts was expanded on gelatin-coated tissue culture surface in medium for ES ce11s in the presence of leukemia inhibitory factor (LIF). Four days later, in medium for embryonic cells without LIF were generated embryonic bodies. In the future work, these ce11s should be propagated in insulin secretion ce11s by using specific, condition. This is a good way for producing of immunocompatible tissue for transplantation in diabetic recipients. It presents powerful tool for prevention of problems associated with diabetes, like an autoimmune destruction of B cells.

stem cells; diabetes mellitus

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