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Pregled bibliografske jedinice broj: 836289

Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS


Topić Popović, Natalija; Kazazić, Snježana; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelinda
Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS // Environmental research, 152 (2017), 7-16 doi:10.1016/j.envres.2016.09.020 (međunarodna recenzija, članak, znanstveni)


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Naslov
Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS

Autori
Topić Popović, Natalija ; Kazazić, Snježana ; Strunjak-Perović, Ivančica ; Čož-Rakovac, Rozelinda

Izvornik
Environmental research (0013-9351) 152 (2017); 7-16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
MALDI-TOF MS ; environmental bacteria ; fish ; crayfish

Sažetak
Identification of bacteria in aquatic and environmental applications, for monitoring purposes and research, for health assessments and therapy considerations of farmed and free- living aquatic organisms, still relies on conventional phenotypic and biochemical protocols. Although molecular techniques based on DNA amplification and sequencing are finding ways into diagnostic laboratories, they are time-consuming, costly and difficult in the case of multiplex assays. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is a rapid and accurate proteomic method reliable for identification of unknown bacteria to the genus and species level. Upon extension of databases, it will certainly find its position in environmental sciences. The paper presents an overview of the principle of the method, its effectiveness in comparison with conventional and molecular identification procedures, and applicability on environmental and aquatic isolates, discussing its advantages and shortcomings, as well as possible future implementations.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Ustanove:
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Topić Popović, Natalija; Kazazić, Snježana; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelinda
Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS // Environmental research, 152 (2017), 7-16 doi:10.1016/j.envres.2016.09.020 (međunarodna recenzija, članak, znanstveni)
Topić Popović, N., Kazazić, S., Strunjak-Perović, I. & Čož-Rakovac, R. (2017) Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS. Environmental research, 152, 7-16 doi:10.1016/j.envres.2016.09.020.
@article{article, year = {2017}, pages = {7-16}, DOI = {10.1016/j.envres.2016.09.020}, keywords = {MALDI-TOF MS, environmental bacteria, fish, crayfish}, journal = {Environmental research}, doi = {10.1016/j.envres.2016.09.020}, volume = {152}, issn = {0013-9351}, title = {Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS}, keyword = {MALDI-TOF MS, environmental bacteria, fish, crayfish} }
@article{article, year = {2017}, pages = {7-16}, DOI = {10.1016/j.envres.2016.09.020}, keywords = {MALDI-TOF MS, environmental bacteria, fish, crayfish}, journal = {Environmental research}, doi = {10.1016/j.envres.2016.09.020}, volume = {152}, issn = {0013-9351}, title = {Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS}, keyword = {MALDI-TOF MS, environmental bacteria, fish, crayfish} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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