engleski

PHYTOCHEMICAL COMPOSITION OF THE ESSENTIAL OIL OF Armeria canescens (Host) Boiss.

The genus Armeria (Plumbaginaceae) is represented by about 120 species, provided that five species were recorded in the Balkan peninsula: A. alpina (DC.) Willd., A. canescens (Host) Boiss., A. maritima (= A. vulgaris Willd.), A. rumelica Boiss. and A. vandasii Hayek (Buzurović et al., 2015). In the area of Croatian are widespread several species, including research species Armeria canescens (Host) Boiss.. This species is the mesothermal halophytes widespread in Central Asia and the Mediterranean. In Croatia growing within the grassland association (as. Armeria canescentis - Festucetum Trinajstić et Sugar), which builds along with plant species Festuca rupicola and Festuca valesiaca Schl .. These association lawn grows on the surface of sinkholes and karst fields along the Dinara mountains. OBJECTIVES: This phytochemical study refers in the A. canescens (in Croatian „babina svila“), with a specific focus on essential oil compounds A search of the literature dealing with secondary metabolites and morphological aspect of genus Armeria resulted with only a few articles (Buzurović et al., 2015 ; Gourguillon et al., 2015 ; Scassellati et al., 2016).. METHOD / DESIGN: Plant material of Armeria canescens was collected from Tijarica (near Split) Croatia in the summer (July) 2013. Aerial parts of plants were performed in a shady place at room temperature for 10 days. Dried aerial parts of plant material (100 g) were subjected to hydrodistillation for 3 h in Clavenger type apparatus. The obtained essential oil was dried over anhydrous sodium sulphate and 1 µL was used for GC/FID and GC/MS measurements. GC/FID analyses were performed on Varian 3900 gas chromatograph equipped with a flame ionization detector (FID) and VF-5ms capillary. GC/MS analyses were carried out on a Varian Saturn 2000 system equipped with a VF-5ms with similar temperature programmed as in GC. The individual peaks were identified by comparison of their retention indices of n- alkanes to those of authentic samples and literature (Adams, 2007), as well as by comparing their mass spectra with the Wiley 9.0 library (Wiley, New York) and NIST/02 mass spectral database. The percentage composition of the samples was computed from the GC peak areas using the normalization method. RESULTS: Water distilled essential oils from aerial parts of investigated plant have been analysed by GC and GC/MS using VF-5ms capillary column. Total yield of oil was 0.05%, based on dry weight of sample. Thirty-one compounds representing 77.9% of the total oil. The essential oil of A. canescens was characterized by a high concentration of diterpene alcohol phytol (29.2%). Among oxygenated monoterpene piperitone (9.4%) was the major compound, followed by phenol compound p-vinyl guaiacol (7.9%). CONCLUSIONS: Previous research in the species Armeria canescens have included morphological and functional aspects of salt glands (Scassellati et al., 2016.).The present study gives additional knowledge about phytochemical composition of the essential oil on the genus Armeria.

Armeria canescens; essential oils; phytol; piperitone; p-vinyl guaiacol

nije evidentirano