engleski

Microsatellite multiplex method for potential use in Black Slavonian pig breeding

Previous studies on Black Slavonian pig breed were focused on the main coat color MC1R locus and on development of the method for Black Slavonian cross-breeds identification, based on coat color genotype. Microsatellites have been used for population studies in pigs for last 25 years and are convenient markers for evaluating the genetic diversity of pig breeds, for parental analysis and identification of pigs as well as traceability of different pig breeds meat in meat products. We developed a PCR multiplex, containing 9 microsatellites in a single reaction. The method was tested on 50 Black Slavonian pig samples (30 homozygous and 20 heterozygous for MC1R locus). For the 9 microsatellite loci analyzed, 79 alleles were detected. The number of detected alleles was high for all loci, with the number of alleles per locus ranging from 5 for S0026, Sw24 and Swr1941 to 22 alleles for S0005. Our results demonstrate that described MS multiplex method may provide a reasonable amount of genetic information to be suitable for use in Black slavonian pig breeding programs in the future.

pigs ; breeds ; Black Slavonian pigs ; genetics ; microsatellites ; multiplex PCR

Rad je prezentiran na skupu International Symposium "Animal Science Days", Session I: Animal genetic resources and breeding ; održanom od 21.-23.09.2016.g., Ptuj, Slovenija.