engleski

Guidelines for the detection of Babesia and Theileria parasites

The genera Babesia and Theileria (phylum Apicomplexa, order Piroplasmida) are mainly transmitted by Ixodid ticks where the sexual part of their life cycle followed by sporogony take place. They include protozoan parasites that infect erythrocytes of a variety of vertebrate hosts including domestic and wild animals, with some Babesia spp. also infecting humans. Babesia sporozoites transmitted in the tick's saliva during the blood meal directly infect erythrocytes where they asexually multiply to produce pearshaped merozoites in the process of merogony, whereas a pre-erythrocytic schizogonic life stage in leukocytes is found in Theileria and precedes merogony in the erythrocytes. The wide spectrum of Babesia and Theileria species and their dissimilar characteristics with relation to disease severity, transmission, epidemiology and drug susceptibility stress the importance of accurate detection of babesiosis and theileriosis and their causative agents. These guidelines review the main methods currently used for the detection of Babesia and Theileria spp. for diagnostic purposes as well as epidemiological studies involving their vertebrate hosts and arthropod vectors. Serological methods were not included once they do not indicate current infection but rather exposure.

Babesia ; Diagnosis ; PCR

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