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Pregled bibliografske jedinice broj: 83154

Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes


Semlitsch, Thomas; Tillian, Hilmar Manfred; Žarković, Neven; Borović, Suzana; Purtscher, Martin; Hohenwarter, Otmar; Schaur, Rudolf Jeorg
Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes // Anticancer Research, 22 (2002), 3; 1689-1697 (međunarodna recenzija, članak, znanstveni)


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Naslov
Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes

Autori
Semlitsch, Thomas ; Tillian, Hilmar Manfred ; Žarković, Neven ; Borović, Suzana ; Purtscher, Martin ; Hohenwarter, Otmar ; Schaur, Rudolf Jeorg

Izvornik
Anticancer Research (0250-7005) 22 (2002), 3; 1689-1697

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
4-hydroxynonenal; Cem-nkr; Lymphocytes; Phytohemagglutinin; Cell proliferation; Brdu; Mtt;

Sažetak
The purpose of this study was to compare the influence of physiological and supraphysiological concentrations of 4-hydroxynonenal (HNE), a peroxidation product of omega-6-polyunsaturated fatty acids, on the proliferation of malignant CEM-NKR lymphatic leukaemia cells and normal human lymphocytes (HPBM). Furthermore the growth modulating effect of phytohemagglutinin (PHA) on both cell lines was examined. The effects of HNE were monitored 18 hours and 3 days after incubation, using two different DNA-synthesis assays ([H-3]-thymidine- and BrdU- incorporation) and a mitochondrial dehydrogenases activity assay (MTT). On the one hand HNE showed dose-dependent effects on both of the cell lines, while on the other hand a clear difference between the response of CEM-NKR cells and HPBM cells, respectively, to HNE was observed. On CDM-NKR cells, both concentrations of HNE caused significant cytotoxic effects on DNA-synthesis as well as on mitochondrial activity, while in contrast, HNE did not show any significant toxicity to HPBM cells. After 3 days there was even a slight stimulation of DNA synthesis with the physiological concentration of HNE. Furthermore the presence of PHA in the culture medium increased the difference of the response of CEM-NKR and HPBM cells, respectively to HNE.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
00981113
0098101

Ustanove:
Institut "Ruđer Bošković", Zagreb


Citiraj ovu publikaciju:

Semlitsch, Thomas; Tillian, Hilmar Manfred; Žarković, Neven; Borović, Suzana; Purtscher, Martin; Hohenwarter, Otmar; Schaur, Rudolf Jeorg
Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes // Anticancer Research, 22 (2002), 3; 1689-1697 (međunarodna recenzija, članak, znanstveni)
Semlitsch, T., Tillian, H., Žarković, N., Borović, S., Purtscher, M., Hohenwarter, O. & Schaur, R. (2002) Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes. Anticancer Research, 22 (3), 1689-1697.
@article{article, year = {2002}, pages = {1689-1697}, keywords = {4-hydroxynonenal, Cem-nkr, Lymphocytes, Phytohemagglutinin, Cell proliferation, Brdu, Mtt, }, journal = {Anticancer Research}, volume = {22}, number = {3}, issn = {0250-7005}, title = {Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes}, keyword = {4-hydroxynonenal, Cem-nkr, Lymphocytes, Phytohemagglutinin, Cell proliferation, Brdu, Mtt, } }
@article{article, year = {2002}, pages = {1689-1697}, keywords = {4-hydroxynonenal, Cem-nkr, Lymphocytes, Phytohemagglutinin, Cell proliferation, Brdu, Mtt, }, journal = {Anticancer Research}, volume = {22}, number = {3}, issn = {0250-7005}, title = {Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes}, keyword = {4-hydroxynonenal, Cem-nkr, Lymphocytes, Phytohemagglutinin, Cell proliferation, Brdu, Mtt, } }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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