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Pregled bibliografske jedinice broj: 830070

Small molecule probes finely differentiate between various ds- and ss-DNA and RNA by fluorescence, CD and NMR response


Crnolatac, Ivo; Rogan, Iva; Majić, Boris; Tomić, Sanja; Deligeorgiev, Todor; Horvat, Gordan; Makuc, Damjan; Plavec, Janez; Pescitelli, Gennaro; Piantanida, Ivo
Small molecule probes finely differentiate between various ds- and ss-DNA and RNA by fluorescence, CD and NMR response // Analytica chimica acta, 940 (2016), 128-135 doi:10.1016/j.aca.2016.08.021 (međunarodna recenzija, članak, znanstveni)


Naslov
Small molecule probes finely differentiate between various ds- and ss-DNA and RNA by fluorescence, CD and NMR response

Autori
Crnolatac, Ivo ; Rogan, Iva ; Majić, Boris ; Tomić, Sanja ; Deligeorgiev, Todor ; Horvat, Gordan ; Makuc, Damjan ; Plavec, Janez ; Pescitelli, Gennaro ; Piantanida, Ivo

Izvornik
Analytica chimica acta (0003-2670) 940 (2016); 128-135

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA/RNA recognition ; fluorescence ; induced circular dichroism ; chiral recognition ; molecular modelling

Sažetak
Two small molecules showed intriguing properties of analytical multipurpose probes, whereby one chromophore gives different signal for many different DNA/RNA by application of several highly sensitive spectroscopic methods. Dyes revealed pronounced fluorescence ratiomeric differentiation between ds-AU-RNA, AT-DNA and GC-DNA in approximate order 10:8:1. Moreover, the dyes showed specific fluorimetric response for poly rA among other ss-RNA. In parallel, the dyes showed specific chiral recognition of poly rU in respect to the other ss-RNA by induced CD (ICD) pattern, which was attributed to the dye-side-chain contribution to binding (confirmed by absence of any ICD band for reference compound lacking side-chain). Most intriguingly, minor difference in the side-chain attached to dye chromophore resulted in opposite sign of dye-ICD pattern, whereby differences in NMR NOESY contacts and proton chemical shifts between two dye/oligo rU complexes combined with MD simulations and CD calculations attributed observed bisignate ICD to the dimeric dye aggregate within oligo rU.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-1477 - Višenamjensko očitavanje DNA / RNA sekundarne strukture molekularnim kemijskim senzorima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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