engleski

The Effect of Di-rahmnolipid BAC-3 on the Healing of Burn Wounds

Di-rhamnolipid BAC-3, secreted into environment by Pseudomonas aeruginosa, at a concentration of 50 μg/ml BAC-3 stimulates the formation of differentiated keratinocyte colonies 34% above the untreated control. The same di-rhamnolipid concentration inhibited fibroblast proliferation 23 % below the control. The treatment of full thickness-burn wounds covering 5% of the whole body skin area with topical applications (2 per day) of 500 μg BAC-3 in eucerine ointment accelerated the closure of wounds on day 21 of the treatment by 32% compared to the control. On day 35, the wounds closed in all animals treated with 500 μg/ml BAC-3 while some rats in the control group had open wounds on days 35 and even 45. Histologic comparisons have shown that the same BAC-3 concentration (500 μg/ml) significantly decreased the extent of fibrosis in burn wounds (47.5%) as compared to the vehicle treated (control) wounds. Results of this study raise the possibility of potential efficacy of BAC-3 in accelerating normal wound healing and perhaps in overcoming defects associated with healing failure in chronic wounds.

keratinocytes; fibroblasts; tissue culture; Di-rahmnolipid BAC-3; wound healing

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