engleski

The kappa-opioid agonist U-69593 affect intracellular calcium level in R1.1 mouse thymoma cell line

In this study the effect of the kappa-opioid agonist U-69593 on the intracellular calcium level in R1.1. cells was investigated using FURA 2-AM dye. In the previous study, calcium transport into R1.1 cells was not affected by the kappa-opioid agonist (-) U50, 488 (Martin-Kleiner and Bidlack, 1999). In this study, the kappa-opioid agonist U-69593 (10-10 M - 10-6 M), decreased intracellular calcium level in unstimulated cells. This decrease could not be reversed by the kappa-opioid antagonist NBI (10-5 M or 10-6 M). Ionophore A23187 was used to increase intracellular calcium level. Stimulation of intracellular calcium level by Ionophore A23187 was potentiated by the kappa-opioid agonist U-69593. Thus, we have shown that basal intracellular calcium level was decreased in R1.1 by the kappa-opioid agonist U-69593 and increased in R1.1 cells stimulated by Ionophore A23187.

intracellular calcium ; kappa-opioids ; mouse thymoma

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