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Tripeptide hydrogelator (Ac-FFA-NH2) as a model of binding site Aβ-protein ; comparative binding studies with Thioflavin-T and other Aβ- binders


Pospišil, Tihomir; Žinić, Mladen; Frkanec, Leo
Tripeptide hydrogelator (Ac-FFA-NH2) as a model of binding site Aβ-protein ; comparative binding studies with Thioflavin-T and other Aβ- binders // SysChem 2016, Abstract Book / Radmila Řápková, Pavel Drašar (ur.).
Prag: Chemicke listy, Association of Czech Chemical Societies,, 2016. str. 126-126a (predavanje, međunarodna recenzija, sažetak, znanstveni)


Naslov
Tripeptide hydrogelator (Ac-FFA-NH2) as a model of binding site Aβ-protein ; comparative binding studies with Thioflavin-T and other Aβ- binders

Autori
Pospišil, Tihomir ; Žinić, Mladen ; Frkanec, Leo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SysChem 2016, Abstract Book / Radmila Řápková, Pavel Drašar - Prag : Chemicke listy, Association of Czech Chemical Societies,, 2016, 126-126a

Skup
SysChem 2016, CMST COST Action CM1304 Emergence and Evolution of Complex Chemical Systems

Mjesto i datum
Valtice Chateau, Čehoslovačka, 8.-12. 05. 2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aβ-amyloid aggregates; Supramolecular self assembly; Low molecular weight peptidic gelators; Binding of histological dyes

Sažetak
Series of tripeptide FFA derivatives was synthesized and tested for gelation of water and organic solvents. Only Ac-FFA-NH2 tripeptide exhibited gelation of water by self- assembly under physiological conditions. TEM of the Ac- FFA-NH2 hydrogel and the methanol/water gel showed the presence of straight fibers with relatively uniform diameters of around 50 nm. FTIR and NMR investigation pointed toward the cross-β structure type of hydrogen bonding of the tripeptide in the gel aggregates. Conjugated dyes (Thioflavin T and Congo Red) are commonly used to stain the plaques in histopathological studies3. Fluorescence titration of Ac-FFA-NH2 aqueous solution bellow its minimal gelation concentration with Thioflavin T (ThT) showed increase of ThT emission with increased tripeptide concentration and formation of the 1 : 1 complex with significant association constant.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
098-0982904-2912 - Samo-udruživanje u gelovima i sinteza funkcionalnih hibridnih materijala (Leo Frkanec, )
HRZZ-IP-2013-11-7387 - Supramolekulska sinteza samo-organizirajućih funkcionalnih nanomaterijala i kompleksnih kemijskih sustava (Leo Frkanec, )

Ustanove
Institut "Ruđer Bošković", Zagreb