engleski

Dysregulation of seryl-tRNA synthetase in kidney disease

Aminoacyl-tRNA synthetases drive cellular protein translation by catalyzing ligation of cognate tRNA with amino acid for use in ribosomal protein synthesis. Recent evidence suggests that aminoacyl-tRNA synthetases exhibit remarkable functional versatility and their noncanonical functions have been implicated in a variety of human diseases including cancers. Vertebrate seryl-tRNA synthetase (SerRS) can enter nucleus where it regulates expression of vascular endothelial growth factor A (VEGFA). VEGFA itself is a key regulator of angiogenesis and SerRS was shown to be essential for development of vertebrate circulatory system. Because SerRS is important in angiogenesis, it could be involved in the process of carcinogenesis. Indeed, SerRS differential expression was shown in prostate cancer. In the present study, both mRNA and protein expression patterns of SerRS were examined in another type of urological cancer, renal cell carcinoma (RCC). Paired clinical specimens (tumor and matched adjacent healthy tissues) were collected from 34 patients. SerRS mRNA expression was examined using RT-qPCR. Our data indicate elevated SerRS expression in 70% of tumor samples. To investigate whether SerRS was also elevated at the protein level, selected paired RCC samples were analized by western blot. The protein level of SerRS in majority of tumor tissues was higher than that in adjacent healthy tissues. The future studies will aim to address clinical significance and biological role of SerRS dysregulation in kidney disease.

aminoacyl-tRNA synthetase ; seryl-tRNA synthetase ; noncanonical function ; VEGFA ; kidney

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