engleski

Alpha-lipoic acid improve decreased regeneration capacity od diabetic slow skeletal muscle

Increased oxidative stress and impaired antioxidant defence are important mechanisms in the pathogenesis of diabetic neuromyopathy. Since diabetes mellitus type 1 decreases muscle regeneration capacity the present study was designed to determine the influence of alpha-lipoic acid (LA), a potent biological antioxidant, on the process of regeneration of diabetic rat skeletal muscles. The regeneration process was provoked in streptozotocin induced diabetic rats in slow (m.soleus, SOL) skeletal muscles by intramuscular injection of myotoxin bupivacain. At intervals of ten days and four weeks, muscle histochemical and morphometrical analysis (fibre cross areas and fibre type distribution) was performed. Changes induced by diabetes are evident in redistribution of muscle fibers and in significant level of atrophy. After four weeks of diabetes, type II muscle fibers showed higher percentage in respect to control group. Slow muscle treated with LA showed a complete recovery with significant higher percentage of type I fibers. Muscle atrophy is present in all fiber types of slow skeletal muscle. Treatment with LA induced significant increase in cross-sectional area in both fiber types. Our results have shown that treatment with LA can reduce changes in the morphological properties caused by diabetes mellitus in slow rat skeletal muscles during the process of regeneration. Treatment with LA during four weeks has shown effects on the redistribution of muscle fibers, and can prevent atrophy in slow diabetic skeletal muscle.

diabetes mellitus; skeletal muscle; regeneration; alpha-lipoic acid

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