Molecular characterization of class B carbapenemases in advanced stage of dissemination and emergence of class D carbapenemases in Enterobacteriaceae from Croatia (CROSBI ID 230258)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Bedenić, Branka ; Sardelić, Sanda ; Luxner, Josefa ; Bošnjak, Zrinka ; Varda-Brkić, Dijana ; Lukić-Grlić, Amarela ; Mareković, Ivana ; Frančula-Zaninović, Sonja ; Krilanović, Marija ; Šijak, Dorotea ; Grisold, Andrea ; Zarfel, Gernot
engleski
Molecular characterization of class B carbapenemases in advanced stage of dissemination and emergence of class D carbapenemases in Enterobacteriaceae from Croatia
Carbapenemases involved in acquired carbapenem resistance in Enterobacteriaceae belong to Ambler class A serin β-lactamases, class B metallo-β-lactamases (MBL) or class D OXA-48- like β-lactamases. The aim of the present study was to analyse the molecular epidemiology and the mechanisms and routes of spread of class B and class D carbapenemases in Croatia. In total 68 isolates were analyzed. Antibiotic susceptibility was determined by broth microdilution method. PCR was used to detect antibiotic-resistance genes. Genotyping was performed by rep-PCR and MLST. Sixty-five isolates were found to harbour VIM-1 carbapenemase, seven of which were positive also for NDM-1, while two strains harboured only NDM-1. OXA-48 was detected in three isolates, two of which coproduced VIM-1. Thirty-six strains possessed additional CTX-M- 15 β-lactamase whereas 64 were positive for TEM-1. CMY was found in 18 C. freundii isolates and DHA-1 in one E. cloacae isolate. Four different plasmid-incompatibility groups were found: A/C, L/M, N and FIIAs. Unlike C. freundii and E. cloacae, K. pneumoniae showed high diversity of rep-PCR patterns. E. cloacae and C. freundii predominantly belonged to one large clone which was allocated to ST105 and ST24, respectively. Three different types of carbapenemases were identified showing the complexity of CRE in Croatia.
VIM-1 ; NDM-1 ; OXA-48 ; Enterobacteriaceae ; resistance
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Podaci o izdanju
43
2016.
74-82
objavljeno
1567-1348
1567-7257
10.1016/j.meegid.2016.05.011
Povezanost rada
Temeljne medicinske znanosti