engleski

Activity of some enzymes in the blood plasma of Hylla rabbits fed various proportions of substrate from the production of the Pleurotus pulmonarius mushrooms

The nutritive effect of different amounts of substrate from the production of Pleurotus pulmonarius on the activity of AST, ALT, LD, CK, GGT and AP in the blood plasma of rabbits, after a nine weeks experimental fattening, was investigated. Sixty male Hyla rabbits, divided in four groups equal in number, one control (C) and three experimental groups (E1, E2, E3), were included in experiment. The control group (C) was fed the standard fodder mix without substrate. Added to the standard mix, the groups E1, E2 and E3 received various proportions, 10%, 20%, and 30%, respectively, of the substrate left from the production of Pleurotus pulmonarius. Different proportions of added substrate did not affect the activity of AST and GGT, respectively, whose values (x &#177; s.d) in the control group ( C ) amounted to 32.49 &#177; 12.8 U.1-1 and 6.14&#177; 1.35 U.l-1 , respectively. At the same time the activity of ALT was altered by experimental diets. The activity of ALT in the control group ( C ) amounted to 28.0&#177; 8.81 U.l-1, whereas in the E3 group it was significantly greater (P<0.05) than in the group C and E2 i.e. 38.86&#177; 11.95 U.l-1. Under the conditions of experimental feeding the activity of AP was greater too: in the control group ( C ) it amounted to 107&#177; 42.5 U.l-1, but in the group E3 it was significantly greater (P<0.05) and amounted to 158.7&#177; 27.7 U.l-1. On the contrary, the activity of CK is decreased during the experimental feeding (P<0.07) and in the control group ( C ) it amounted to 2044 &#177; 647 U.l-1, in the E3 group to 1479.6&#177; 927 U.l-1. The activity of lactate dehydrogenase (LD) generally reflected the composition of fattening diets and is inversely proportionate to the share of substrate added. The highest values of LD activity was found in group C (94.57&#177; 24.78 U.l-1), and the lowest was in group E3 (63.49&#177; 14.12 U.l-1). The difference was significant compared to the C and E1, groups (P<0.01) and to the E2 (P<0.05) group. The results indicate B1 hypovitaminosis and the development of compensatory metabolic acidosis.

Nutrition; substrate of mushrooms; aspartate aminotransferase (AST); alanine aminotransferase (ALT); lactate dehydrogenase (LD); creatine kinase (CK); gamma-glutamyltransferase (GGT); alkaline phosphatase (AP)1; hypovitaminosis; metabolic acidosis

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