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Pregled bibliografske jedinice broj: 825805

Perinatal mouse cytomegalovirus infection induces activation of brain resident microglial cells and recruitment of inflammatory cells into the brain


Kveštak, Daria; Brizić, Ilija; Arapović, Jurica; Arapović, Maja; Huszthy, Peter Csaba; Šušak, Božo; Golemac, Mijo; Pernjak Pugel, Ester; Torti, Nicole; Oxenius, Annette et al.
Perinatal mouse cytomegalovirus infection induces activation of brain resident microglial cells and recruitment of inflammatory cells into the brain // 14th Symposium Translation of basic immunology and neuroscience tools to therapies: Where are we now?
Rijeka, Hrvatska, 2016. (poster, nije recenziran, sažetak, znanstveni)


Naslov
Perinatal mouse cytomegalovirus infection induces activation of brain resident microglial cells and recruitment of inflammatory cells into the brain

Autori
Kveštak, Daria ; Brizić, Ilija ; Arapović, Jurica ; Arapović, Maja ; Huszthy, Peter Csaba ; Šušak, Božo ; Golemac, Mijo ; Pernjak Pugel, Ester ; Torti, Nicole ; Oxenius, Annette ; Krmpotić, Astrid ; Britt, William J ; Jonjić, Stipan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
14th Symposium Translation of basic immunology and neuroscience tools to therapies: Where are we now?

Mjesto i datum
Rijeka, Hrvatska, 2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Prirođena citomegalovirusna infekcija; mikroglija; NK stanice; CD8+ T limfociti
(Congenital cytomegalovirus infection; microglia; NK cells; CD8+ T cells)

Sažetak
Congenital human cytomegalovirus (HCMV) infection is the most common viral cause of long-term neurodevelopmental sequelae, including microcephaly and sensorineural hearing loss. As HCMV does not cross the species barrier, we employed a mouse model in which newborn mice are infected by intraperitoneal (i.p.) inoculation of mouse cytomegalovirus (MCMV). Following infection the virus disseminates to the central nervous system (CNS) and replicates in the brain parenchyma. CNS infection leads to the activation of resident microglial cells and the recruitment of peripheral immune cells. In addition, the virus induces delay in cerebellar growth. In our model of congenital MCMV infection, the initial neuroimmune responses are dominated by activation of resident microglial cells and the influx of NK cells, whose appearance coincides with detection of the virus in the brain. The number of NK cells in the CNS peaked at day 8 post infection (p.i.). In addition, we also observed recruitment of other peripheral immune cells, of which CD8+ T cells were the most numerous and peaked on day 21 p.i. Phenotypic analysis showed that MCMV-specific CD8+ T cells are highly activated and display tissue resident memory phenotype during latency. Our results demonstrated the coordinated action of innate and acquired immunity in the clearance of congenital MCMV infection of the brain. Furthermore, the results demonstrated the functional role of antigen-specific CD8+ T cells in protection from virus-induced pathology in the neonatal CNS.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekt / tema
062-0621261-1269 - Perinatalni citomegalovirusni encefalitis (Ester Pernjak-Pugel, )

Ustanove
Medicinski fakultet, Rijeka

Citiraj ovu publikaciju

Kveštak, Daria; Brizić, Ilija; Arapović, Jurica; Arapović, Maja; Huszthy, Peter Csaba; Šušak, Božo; Golemac, Mijo; Pernjak Pugel, Ester; Torti, Nicole; Oxenius, Annette et al.
Perinatal mouse cytomegalovirus infection induces activation of brain resident microglial cells and recruitment of inflammatory cells into the brain // 14th Symposium Translation of basic immunology and neuroscience tools to therapies: Where are we now?
Rijeka, Hrvatska, 2016. (poster, nije recenziran, sažetak, znanstveni)
Kveštak, D., Brizić, I., Arapović, J., Arapović, M., Huszthy, P., Šušak, B., Golemac, M., Pernjak Pugel, E., Torti, N. & Oxenius, A. (2016) Perinatal mouse cytomegalovirus infection induces activation of brain resident microglial cells and recruitment of inflammatory cells into the brain. U: 14th Symposium Translation of basic immunology and neuroscience tools to therapies: Where are we now?.
@article{article, year = {2016}, keywords = {congenital cytomegalovirus infection, microglia, NK cells, CD8+ T cells}, title = {Perinatal mouse cytomegalovirus infection induces activation of brain resident microglial cells and recruitment of inflammatory cells into the brain}, keyword = {congenital cytomegalovirus infection, microglia, NK cells, CD8+ T cells}, publisherplace = {Rijeka, Hrvatska} }