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Distribution of the organic anion transporters NaDC3 and OAT1-3 along the human nephron


Breljak, Davorka; Ljubojević, Marija; Hagos, Yohannes; Micek, Vedran; Balen Eror, Daniela; Vrhovac Madunić, Ivana; Brzica, Hrvoje; Karaica, Dean; Radovic, Nikola; Kraus, Ognjen et al.
Distribution of the organic anion transporters NaDC3 and OAT1-3 along the human nephron // American journal of physiology. Renal physiology, 311 (2016), 1; F227-F238 doi:10.1152/ajprenal.00113.2016 (međunarodna recenzija, članak, znanstveni)


Naslov
Distribution of the organic anion transporters NaDC3 and OAT1-3 along the human nephron

Autori
Breljak, Davorka ; Ljubojević, Marija ; Hagos, Yohannes ; Micek, Vedran ; Balen Eror, Daniela ; Vrhovac Madunić, Ivana ; Brzica, Hrvoje ; Karaica, Dean ; Radovic, Nikola ; Kraus, Ognjen ; Naohiko, Anzai ; Koepsell, Hermann ; Burckhardt, Gerhard ; Burckhardt, Birgitta Christina ; Sabolić, Ivan.

Izvornik
American journal of physiology. Renal physiology (1931-857X) 311 (2016), 1; F227-F238

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Na+-K+-ATPase ; Na+-dicarboxylate cotransporter 3 ; Western blotting ; human kidney ; immunocytochemistry ; organic anion transporter 1 ; organic anion transporter 2 ; organic anion transporter 3 ; sex differences ; species differences

Sažetak
Abstract The initial step in renal secretion of organic anions (OA) is mediated by transporters in the basolateral membrane (BLM). Contributors to this process are the primary-active Na+/K+- ATPase (EC 3.6.3.9), the secondary-active sodium-dicarboxylate cotransporter 3 (NaDC3/SLC13A3), and the tertiary-active OA transporters OAT1/SLC22A6, OAT2/SLC22A7 and OAT3/SLC22A8. In human kidneys we analyzed the localization of these transporters by immunochemical methods in tissue cryosections and isolated membranes. The specificity of antibodies was validated with HEK293 cells stably transfected with functional OA transporters. The Na+/K+-ATPase was immunolocalized to the BLM along the entire human nephron. NaDC3-related immunostaining was detected in the BLM of proximal tubules and in the BLM and/or luminal membrane of principal cells in connecting segments and collecting ducts. The thin and thick ascending limbs, macula densa, and distal tubules exhibited no reactivity with the anti-NaDC3 antibody. In OAT1-3-related immunostaining in human kidneys was detected only in the BLM of cortical proximal tubules ; all three OATs were stained more intensely in S1/S2 segments as compared to S3 segment in medullary rays, whereas the S3 segment in outer stripe remained unstained. The expression of NaDC3, OAT1, OAT2, and OAT3 proteins exhibited considerable interindividual variability in both male and female kidneys, and sex differences in their expression could not be detected. Our studies provide a side-by- side comparison of basolateral transporters co- operating in renal OA secretion in the human kidney.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-1481 - Starosno-ovisna ekspresija membranskih prijenosnika u štakora (Ivan Sabolić, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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