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Pregled bibliografske jedinice broj: 824312

Interplay of proteins involved in translation and steroid hormone metabolism in Arabidopsis


Rokov Plavec, Jasmina; Kekez, Mario; Zanki, Vladimir; Bauer, Nataša
Interplay of proteins involved in translation and steroid hormone metabolism in Arabidopsis // Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology on the Occasion of the 40th Anniversary, HDBMB2016 / Katalinić, Maja ; Kovarik, Zrinka (ur.).
Zagreb: Grafokor d.o.o., 2016. str. 49-49 (pozvano predavanje, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 824312 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Interplay of proteins involved in translation and steroid hormone metabolism in Arabidopsis

Autori
Rokov Plavec, Jasmina ; Kekez, Mario ; Zanki, Vladimir ; Bauer, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology on the Occasion of the 40th Anniversary, HDBMB2016 / Katalinić, Maja ; Kovarik, Zrinka - Zagreb : Grafokor d.o.o., 2016, 49-49

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology on the Occasion of the 40th Anniversary, HDBMB2016

Mjesto i datum
Split, Hrvatska, 1-4.6.2016

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
aminoacyl-tRNA synthetase ; seryl-tRNA synthetase ; protein-protein interactions ; plant ; brassinosteroids ; localization

Sažetak
The rules of genetic code are established by aminoacyl-tRNA synthetases (aaRSs), enzymes that covalently link tRNA with its cognate amino acid. Many aaRSs are involved in diverse cellular processes beyond translation by forming protein- protein interactions. However, the studies of plant aaRS assemblies are scarce. Recently, we identified BEN1 as protein interactor of Arabidopsis seryl-tRNA synthetase (SerRS). BEN1 is a putative oxidoreductase involved in metabolism of steroidal plant hormones brassinosteroids. These plant growth-promoting hormones regulate a variety of physiological processes crucial for normal plant growth and development and are also involved in plant stress response. The SerRS:BEN1 complex is the first protein complex of plant aaRSs discovered so far and also one of the rare examples of interaction of aaRSs with enzymes involved in primary or secondary metabolism. To pinpoint regions responsible for interaction, truncated variants of both SerRS and BEN1 were prepared and analyzed using microscale thermophoresis, that enables KD determination. Detailed analysis showed that interaction interface involves SerRS globular catalytic domain and acidic N-terminus of BEN1. Moreover, first 17 amino acids of BEN1 is necessary, but not sufficient for interaction, implying that other parts of BEN1 also participate in interaction with SerRS. Furthermore, using RT-qPCR we observed that expression of BEN1 gene in transgenic plants overexpressing SerRS is slightly lower compared to wild type. To test whether SerRS regulates BEN1 expression in nucleus we determined its localization. Our data show that SerRS is exclusively localized in cytosol, despite its several putative nuclear localization signals. We also investigated the role of SerRS in stress using transgenic plants overexpressing SerRS. Our results show that transgenic seeds germinate earlier and that in most cases transgenic seedlings grow better on various stress media compared to wild type seedlings. This indicates that enhanced expression of SerRS plays a role in the response of Arabidopsis to abiotic stress conditions. Considering that brassinosteroids are also involved in plant stress response we discuss functional importance of SerRS:BEN1 assembly.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
HRZZ-09.01/293 - Nekanonske uloge aminoacil-tRNA-sintetaza (Gruić Sovulj, Ita, HRZZ ) ( POIROT)
MZOS-119-0982913-1358 - Strukturna raznolikost seril-tRNA sintetaza i točnost biosinteze proteina (Rokov Plavec, Jasmina; Weygand Đurašević, Ivana, MZOS ) ( POIROT)
EK-FP7-315997 - Integrating research in molecular life sciences at the University of Zagreb (INTEGRA-LIFE) (Lauc, Gordan; Zoldoš, Vlatka; Gruić-Sovulj, Ita; Vlahoviček, Kristian; Weygand Đurašević, Ivana; Rokov Plavec, Jasmina; Maravić Vlahoviček, Gordana, EK - FP7-REGPOT-2012-2013-1) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Mario Kekez (autor)

Avatar Url Nataša Bauer (autor)

Avatar Url Vladimir Zanki (autor)

Avatar Url Jasmina Rokov Plavec (autor)


Citiraj ovu publikaciju:

Rokov Plavec, Jasmina; Kekez, Mario; Zanki, Vladimir; Bauer, Nataša
Interplay of proteins involved in translation and steroid hormone metabolism in Arabidopsis // Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology on the Occasion of the 40th Anniversary, HDBMB2016 / Katalinić, Maja ; Kovarik, Zrinka (ur.).
Zagreb: Grafokor d.o.o., 2016. str. 49-49 (pozvano predavanje, domaća recenzija, sažetak, znanstveni)
Rokov Plavec, J., Kekez, M., Zanki, V. & Bauer, N. (2016) Interplay of proteins involved in translation and steroid hormone metabolism in Arabidopsis. U: Katalinić, M. & Kovarik, Z. (ur.)Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology on the Occasion of the 40th Anniversary, HDBMB2016.
@article{article, author = {Rokov Plavec, Jasmina and Kekez, Mario and Zanki, Vladimir and Bauer, Nata\v{s}a}, year = {2016}, pages = {49-49}, keywords = {aminoacyl-tRNA synthetase, seryl-tRNA synthetase, protein-protein interactions, plant, brassinosteroids, localization}, title = {Interplay of proteins involved in translation and steroid hormone metabolism in Arabidopsis}, keyword = {aminoacyl-tRNA synthetase, seryl-tRNA synthetase, protein-protein interactions, plant, brassinosteroids, localization}, publisher = {Grafokor d.o.o.}, publisherplace = {Split, Hrvatska} }
@article{article, author = {Rokov Plavec, Jasmina and Kekez, Mario and Zanki, Vladimir and Bauer, Nata\v{s}a}, year = {2016}, pages = {49-49}, keywords = {aminoacyl-tRNA synthetase, seryl-tRNA synthetase, protein-protein interactions, plant, brassinosteroids, localization}, title = {Interplay of proteins involved in translation and steroid hormone metabolism in Arabidopsis}, keyword = {aminoacyl-tRNA synthetase, seryl-tRNA synthetase, protein-protein interactions, plant, brassinosteroids, localization}, publisher = {Grafokor d.o.o.}, publisherplace = {Split, Hrvatska} }




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