engleski

Seroprevalence of blood borne pathogens among Croatian solid organ donors 2006-2014

Background: The success of transplantation and graft survival are closely related to the absence of infection of recipient by infected graft, reactivated recipient's latent infections, nosocomial infections and community acquired infections. Since 2006, the Croatian Institute of Transfusion Medicine has been providing mandatory testing of solid organ donors (SOD) for blood borne pathogens (24/7) for the entire country. In 2013 molecular testing of SOD, ID-NAT for HBV-DNA, HCV-RNA and HIV1-RNA, was introduced. Aims: To estimate the seroprevalence of blood borne pathogens among Croatian SOD, quality of samples measured against rate of dilution and turnaround time. Methods: In the period from 5/11/2006 to 31/12/2014 a total of 944 solid organ donor’s plasma samples were tested by means of Abbott Architect and bioMerieux Vidas tests, for HIV, HBV, HCV, CMV, EBV, T. pallidum (anti-TP) and T. gondii (Toxo). Each positive result for HBsAg, anti-HCV, isolatedanti-HBc and IgM (EBV VCA, CMV and Toxo) was retested on alternative analyzer/test (Architect i2000SR or Vidas). Anti-TP positive result was retested and additionally tested for TPHA and RPR test. Results: Seroprevalence of EBV, CMV and T. gondii among SOD was as high as expected, 97%, 92% and 70%, respectively. There was no HIV positive SOD, 0.6% were HBsAg positive, 10.6% anti-HBc, 1.4% anti-HCV and 1% anti-TP. Isolated anti-HBc positive result, as possible marker of occult hepatitis B virus infection (OBI), was detected in 3% of tested donors. 14% were only anti-HBs positive, probably due to vaccination. 2.5% samples were diluted more than 50%. Average testing turnaround time was 2 hours. Conclusions: The results indicate the adequacy of the SOD selection, tests used and testing algorithms. Seroprevalence of HIV, HBV, HCV and T. pallidum is low and expected as it is high seroprevalence of ubiquitous pathogens suggesting the appropriate sensitivity of solid organ donor's selection methods in donor hospitals.

Solid Organ Donors; Blood Borne Pathogens

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