engleski

Anaerobic digestion of brewery spent grain as a mono-substrate in a two-stage anaerobic digestion using solid-state digestion reactor and granulated biomass reactor

Anaerobic digestion of brewery spent grain as a mono-substrate was studied. Anaerobic digestion was phase separated in solid state anaerobic digestion reactor, where mostly microbiological hydrolysis and acidogenesis and granular biomass reactor where mostly methanogenesis was performed. The overall process exhibited total solids degradation efficiency between 73.6 and 80.4%. Average specific biogas production was 424±36 L/kg, whereas biomethane production was 230±34 L/kg of brewery spent grain total solids. Granular biomass after adaptation exhibited stabile operation at C-N ratios as low as 0.2 – 0.3, which is rare in anaerobic digestion. P-cresol as a degradation product was present in concentrations up to 45 mg/L and during the process successfully degraded. The excellent adaptability of the granular biomass is confirmed by 67% shift in bacterial and a 32% shift in archaeal community structure in granular biomass reactor after 198 days of successful operation.

Anaerobic digestion ; biogas production ; brewery spent grain ; microbial biomass ; p-cresol degradation ; solid state anaerobic digestion

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