engleski

Lipid peroxidation after imidacloprid incubation on F11 cellular model

Neuronal excitability is a parameter related to neuron potential to generate signals of novel stimuli. Long-term exposure to environmental chemicals is believed to be a predisposing factor for further establishment of altered neuronal functions that often can be translated into chronic neuronal diseases. The molecular irreversible events leading chemical exposure to become risk factors for neuronal sensitisation, however are not yet completely understood. Imidacloprid is an insecticide of the neo-nicotinoid family, that operate preferentially via nicotinic neuronal receptors expressed on both invertebrates and mammalian cells. In this work, we explored the effects of imidacloprid (0.1 - 4 mM) on F11 neuronal cell cultures. Cells were analysed for mitochondrial activity with MTT assay, in vivo mitochondrial staining and reactivity of lipid peroxidation enzymes involved in stress reactions. These results contribute to new assessment of imidacloprid effects on mammalian tissue.

lipid peroxidation; neonicotinoids; neuronal cell model

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