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Presence of bacterium Acinetobacter baumannii in wastewaters of the City of Zagreb


Hrenović, Jasna; Goić-Barišić, Ivana; Kazazić, Snježana; Hunjak, Blaženka; Stipaničev, Draženka; Repec, Siniša; Ganjto, Marin
Presence of bacterium Acinetobacter baumannii in wastewaters of the City of Zagreb // 6th Croatian Congress of Microbiology with International Participation Book of Abstracts / Antolović, Roberto (ur.).
Zagreb: Croatian Microbiological Society, 2016. str. 71-72 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Presence of bacterium Acinetobacter baumannii in wastewaters of the City of Zagreb

Autori
Hrenović, Jasna ; Goić-Barišić, Ivana ; Kazazić, Snježana ; Hunjak, Blaženka ; Stipaničev, Draženka ; Repec, Siniša ; Ganjto, Marin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Croatian Congress of Microbiology with International Participation Book of Abstracts / Antolović, Roberto - Zagreb : Croatian Microbiological Society, 2016, 71-72

ISBN
978-953-7778-13-2

Skup
6th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 15-18. 06. 2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Hospital ; wastewater ; carbapenems ; pathogen ; epidemiology

Sažetak
Acinetobacter baumannii is an emerging hospital pathogen causing outbreaks in Croatia since 2002 and is still present in Croatian hospitals. Clinical isolates of A. baumannii in Croatian hospitals are usually multi-drug resistant(MDR), with resistance to carbapenems dramatically increasing from 10% in 2008 to 82% in 2014. Although A. baumannii has been isolated from patients and hospital environment during outbreaks, crucial questions regarding its epidemiology remains incompletely understood. The aim of this study was to screen the hospital and municipal wastewater of the City of Zagreb for the presence of viable A. baumannii and carbapenems. Sampling of hospital wastewater was performed on 2 occasions in 2015 at the central manhole of one Zagreb’s hospital from which the clinical isolates of A. baumannii were recovered. Sampling of municipal wastewater was performed on 6 occasions in 2014/15 at the influent and effluent of the central Zagreb’s wastewater treatment plant. Concentration of carbapenems in wastewater was measured by UHPLC Q-TOF MS. The isolation of A. baumannii from wastewater was performed at 42C/48h on CHROMagar Acinetobacter without or with the addition of commercial supplement CR102 which allows the growth of carbapenem- resistant isolates. Presumptive A. baumannii colonies were characterized phenotypically, by using Vitek2 system, and MALDI-TOF MS. Antibiotic resistance profiles were determined by Vitek2 system and interpreted according to EUCAST criteria. Hospital and municipal wastewaters contained high concentrations of imipenem and meropenem which averaged: 894 and 129ng/L in hospital wastewater, 3060 and 380ng/L in raw and 497 and 311ng/L in treated municipal wastewater, respectively. From hospital wastewater, raw and treated municipal wastewater 8, 30 and 7 isolates of A. baumannii were recovered, respectively. All isolates from hospital wastewater and majority (33/37) of isolates from municipal wastewater were resistant to carbapenems and majority of tested antibiotics except colistin. The 7 MDR clinical isolates recovered in the same period showed comparable levels of antibiotic resistance to MDR isolates from hospital and municipal wastewater. These suggest that A. baumannii is able to survive in environment outside hospitals. However, 4 isolates from raw municipal wastewater were susceptible to carbapenems and other antibiotics. This finding opens the possibility that A. baumannii could have natural habitat in sewage system.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
IP-2014-09-5656

Ustanove
Hrvatski zavod za javno zdravstvo,
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
KBC Split,
Medicinski fakultet, Split

Citiraj ovu publikaciju

Hrenović, Jasna; Goić-Barišić, Ivana; Kazazić, Snježana; Hunjak, Blaženka; Stipaničev, Draženka; Repec, Siniša; Ganjto, Marin
Presence of bacterium Acinetobacter baumannii in wastewaters of the City of Zagreb // 6th Croatian Congress of Microbiology with International Participation Book of Abstracts / Antolović, Roberto (ur.).
Zagreb: Croatian Microbiological Society, 2016. str. 71-72 (poster, domaća recenzija, sažetak, znanstveni)
Hrenović, J., Goić-Barišić, I., Kazazić, S., Hunjak, B., Stipaničev, D., Repec, S. & Ganjto, M. (2016) Presence of bacterium Acinetobacter baumannii in wastewaters of the City of Zagreb. U: Antolović, R. (ur.)6th Croatian Congress of Microbiology with International Participation Book of Abstracts.
@article{article, editor = {Antolovi\'{c}, R.}, year = {2016}, pages = {71-72}, keywords = {hospital, wastewater, carbapenems, pathogen, epidemiology}, isbn = {978-953-7778-13-2}, title = {Presence of bacterium Acinetobacter baumannii in wastewaters of the City of Zagreb}, keyword = {hospital, wastewater, carbapenems, pathogen, epidemiology}, publisher = {Croatian Microbiological Society}, publisherplace = {Sveti Martin na Muri, Hrvatska} }