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Survival of Francisella novicida after exposure to drying and different growth conditions


Kukurin, Corina; Matulja, Dario; Mihatović, Ana; Ožanič, Mateja; Marečić, Valentina; Šantić, Marina
Survival of Francisella novicida after exposure to drying and different growth conditions // 6th Croatian Congress of Microbiology with International Participation - Book of Abstract / Antolović, Roberto (ur.).
Zagreb: Croatian Microbiological Society, 2016. str. 74-74 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Survival of Francisella novicida after exposure to drying and different growth conditions

Autori
Kukurin, Corina ; Matulja, Dario ; Mihatović, Ana ; Ožanič, Mateja ; Marečić, Valentina ; Šantić, Marina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Croatian Congress of Microbiology with International Participation - Book of Abstract / Antolović, Roberto - Zagreb : Croatian Microbiological Society, 2016, 74-74

ISBN
978-953-7778-13-2

Skup
6th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Sveti Martin na Muri, Croatia, 15-18.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Francisella; drying; L-cysteine; α–ketoglutarate

Sažetak
Francisella tularensis is a gram-negative facultative intracellular bacterium that can cause a fatal disease tularemia, in human and animals. The genus Francisella contains five species: F. tularensis, F. philomiragia, F. hispaniensis, F. noatunensis and F. novicida. The bacterium has been isolated from wide variety of organisms including fish, bird, rabbits, squirrels, hares, flies and amoebae. It has been speculated that bacterium can persist in the environment without the presence of a suitable host. In this study we investigated the internal and external factors that influence the survival of F. novicida in vitro. The survival of F. novicida was followed after growing the bacteria in buffered yeast extract (BYE) broth with standard concentration of L – cysteine and α – ketoglutarate as well as in BYE broth where concentration of L – cysteine and α – ketoglutarate were changed. Further, the viability of F. novicida was observed after exposure of bacteria to air drying during 60 seconds, 30, 60 and 90 minutes followed by recovering in D. discoideum cells. The growth of F. novicida was significantly enhanced in the presence of high concentration of L – cysteine and α – ketoglutarate in BYE broth. Bacteria exposed to air drying over 60 seconds was able to recover in D. discoideum cells, but the longer periods of drying resulted in damaging the cell viability.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
062-0621273-0950 - Francisella tularensis-unutarstanični život i patogeneza tularemije u miša (Marina Šantić, )

Ustanove
Medicinski fakultet, Rijeka,
Sveučilište u Rijeci - Odjel za biotehnologiju