engleski

Is there any improvement in glycolysis inhibition due to new additive mixture?

Background: Reliable stabilization of blood glucose concentration directly after sampling is significant because it can affect the accuracy of measurement. Therefore, it is necessary to set higher quality standards for preanalytical phase by ensuring effective glycolysis inhibition in order to increase the accuracy of the diagnosis. In this study we wanted to investigate whether new Vacuette Glucomedics tubes (additive: sodium EDTA, sodium fluoride, citric acid and sodium citrate, Greiner Bio-One) are more efficient in immediate and complete inhibition of glycolysis than conventional BD Vacutainer tubes (additive: sodium fluoride and potassium oxalate, Becton–Dickinson) Materials and methods: Venous whole blood was collected from 48 presumably healthy donors by using a Vacuette Glucomedics and BD Vacutainer tubes. The collected sample tubes were centrifuged initially after blood collection and analyzed for glucose (hexokinase method) at the initial time point, 24h and 48h after blood collection on the AU680 analyzer (Beckman Coulter, Tokyo, Japan). Statistical analyses were performed with Mann— Whitney and Kruskal-Wallis tests by using MedCalc 12.2.1.0. statistical software (MedCalc, Mariakerke, Belgium). P < 0.05 was considered statistically significant. Results: Our results showed that glucose concentration was significantly higher at all time points in Vacuette Glucomedics compared to BD Vacutainer tubes (P values were as follows: P(0h) = 0.002 ; P(24h) = 0.002 ; P(48h) = 0.001). On the other hand, there was no significant decline at different times of analysis after blood collection either in Vacuette Glucomedics tubes (P = 0.67) or in BD Vacutainer tubes (P = 0.53). Conclusions: According to our results the stabilization rate of glucose concentration is more effective for Vacuette Glucomedics tubes. Additionally, the glucose concentration of plasma samples in both types of tubes was constant at room temperature for up to 48 hours. These results indicate that Vacuette Glucomedics tubes are more suitable for reliable determination of glucose concentration after blood collection and if prolonged processing time (including transport and/or storage) occurs.

Glucomedics tubes ; glycolysis inhibition ; blood glucose

nije evidentirano