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Pregled bibliografske jedinice broj: 818585

Discovery of ‘click’ 1, 2, 3-triazolium salts as potential anticancer drugs


Steiner, Ivana; Stojanović, Nikolina; Bolje, Aljoša; Brozović, Anamaria; Polančec, Denis; Ambriović-Ristov, Andreja; Radić Stojković, Marijana; Piantanida, Ivo; Eljuga, Domagoj; Košmrlj, Janez; Osmak, Maja
Discovery of ‘click’ 1, 2, 3-triazolium salts as potential anticancer drugs // Radiology and oncology, 50 (2015), 3; 280-288 doi:10.1515/raon-2016-0027 (međunarodna recenzija, članak, znanstveni)


Naslov
Discovery of ‘click’ 1, 2, 3-triazolium salts as potential anticancer drugs

Autori
Steiner, Ivana ; Stojanović, Nikolina ; Bolje, Aljoša ; Brozović, Anamaria ; Polančec, Denis ; Ambriović-Ristov, Andreja ; Radić Stojković, Marijana ; Piantanida, Ivo ; Eljuga, Domagoj ; Košmrlj, Janez ; Osmak, Maja

Izvornik
Radiology and oncology (1318-2099) 50 (2015), 3; 280-288

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Triazoles; triazolium salts; anticancer activity; cell cycle; ROS

Sažetak
Background. In order to increase the effectiveness of cancer treatment, new compounds with potential anticancer activities are synthesized and screened. Here we present the screening of a new class of compounds, 1- (2-picolyl)-, 4-(2-picolyl)-, 1-(2-pyridyl)-, and 4-(2-pyridyl)-3-methyl-1, 2, 3-triazolium salts and ‘parent’ 1, 2, 3-triazole precursors. Methods. Cytotoxic activity of new compounds was determined by spectrophotometric MTT assay on several tumour and one normal cell line. Effect of the selected compound to bind double stranded DNA (ds DNA) was examined by testing its influence on thermal stability of calf thymus DNA while its influence on cell cycle was determined by flow cytometric analysis. Generation of reactive oxygen species (ROS) was determined by addition of specific substrate 5- (and-6)- chloromethyl-2’, 7’- dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA). Results. Parent triazoles were largely inactive, while some of the triazolium salts were highly cytotoxic for HeLa cells. Triazolium salts exhibited high cell- type dependent cytotoxicity against different tumour cells. Selected compound (4- (4- methoxyphenyl)-3-methyl-1-(2-picolyl)-1H-1, 2, 3-triazolium hexafluorophosphate(V) (2b) was significantly more cytotoxic against tumour cells than to normal cells, with very high therapeutic index 7.69 for large cell lung carcinoma H460 cells. Additionally, this compound was similarly cytotoxic against parent laryngeal carcinoma HEp-2 cells and their drug resistant 7T subline, suggesting the potential of this compound in treatment of drug resistant cancers. Compound 2b arrested cells in the G1 phase of the cell cycle. It did not bind ds DNA, but induced ROS in treated cells, which further triggered cell death. Conclusions. Our results suggest that the ‘click’ triazolium salts are worthy of further investigation as anti-cancer agents.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekt / tema
098-0982913-2748 - Stanični odgovor na citotoksične spojeve i razvoj otpornosti (Maja Osmak, )
098-0982913-2850 - Povećanje transdukcije adenovirusnih vektora i otpornost stanica na citostatike (Andreja Ambriović Ristov, )
098-0982914-2918 - Dizajn, sinteza i ispitivanje interakcija malih molekula s DNA, RNA i proteinima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
KBC "Sestre Milosrdnice",
Dječja bolnica Srebrnjak

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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