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Trichoptera research within project “EU Natura 2000 Integration Project –NIP"

Republic of Croatia is very rich in biodiversity which is a result of specific geographical position and climate, geomorphological and hydrogeographical conditions which create wide range of habitats. Unfortunately, a comprehensive inventory of Croatian biodiversity does not exist. A step towards achieving this goal is the project “EU Natura 2000 Integration Project – NIP“. NIP is under jurisdiction of Ministry of Environment and Nature Protection based on the Loan Agreement with the World Bank. One of the components of the project is a systematic inventory of biological diversity of those taxonomic groups which are considered as poorly investigated. One such group were caddisflies (Trichoptera) for which a team of all available national experts was formed in order to do field research and laboratory processing. The plan is to conduct Trichoptera inventory on selected 105 localities in Croatia in two years (2014-2015). In the first year 52 localities have been sampled three (3) times a year in the following periods: March-May, June-August and September-October. Trichoptera were collected during the day by hand and using an entomological net (sweep net) and during the night using UV light-traps with reflective canvas on a pyramidal construction. After the completion of the field survey and data analysis, the checklist of species recorded in Croatia for Trichoptera taxonomic group will be revised. So far field research and laboratory processing have been conducted at 77 localities. We recorded 124 species of Trichoptera. Very interesting finding is 2 potentially new species found at the spring of the Zrmanja River. According to our research, the most abundant species is Rhyacophila fasciata Hagen 1859, recorded at 27 localities. The number of known Trichoptera species in Croatia is 186. This is a very significant number for a relatively small country (56.594 km2). There are, however, still considerable gaps in our knowledge about taxonomy, distribution and ecology of many taxonomic groups, particularly of the invertebrate taxa. New species and subspecies are being discovered each year. A powerful tool for obtaining a more accurate number of species, but also discovering new, is DNA Barcoding. Therefore, among the first in Croatia, we started systematically with DNA Barcoding Trichoptera. Adult Trichoptera specimens collected within NIP project we DNA Barcoded using The Barcode of Life Data Systems (BOLD) methodology for barcoding.

Trichoptera; Croatia

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